217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑10 Ra Monoclonal Antibody (Catalog # MAB274). The ND₅₀ is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL)." class="big_lightbox"> 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑10 Ra Monoclonal Antibody (Catalog # MAB274). The ND₅₀ is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL)." alt="Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑10 Ra Monoclonal Antibody (Catalog # MAB274). The ND₅₀ is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL)." class="big_thumb" />

417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-474-NA). The ND₅₀ is typically 1-3 µg/mL." class="big_lightbox"> 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-474-NA). The ND₅₀ is typically 1-3 µg/mL." alt="Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-474-NA). The ND₅₀ is typically 1-3 µg/mL." class="big_thumb" />

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> Fluorescent ICC Staining of Non-adherent Cells." alt="IL‑10 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Goat Anti-Human IL‑10 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑274‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta /IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secretion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-274-NA). The ND₅₀ is typically 20-40 µg/mL in the presence of LPS (0.25-3 ng/mL)." class="big_lightbox"> 217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta /IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secretion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-274-NA). The ND₅₀ is typically 20-40 µg/mL in the presence of LPS (0.25-3 ng/mL)." class="big_thumb" />

HAF007). A specific band was detected for IL-10 Ra at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for IL-10 Ra at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human IL-10 Ra Monoclonal Antibody (Catalog # MAB2742) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-10 Ra at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0102B) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0102B) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />