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217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑10 Ra Monoclonal Antibody (Catalog # MAB274). The ND50 is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL)." class="big_lightbox" target="_blank">
417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-474-NA). The ND50 is typically 1-3 µg/mL." class="big_lightbox" target="_blank">
217-ILB/CF) is coated at 2 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-10 R alpha Fc Chimera Avi-tag (Catalog # AVI9044) binds with an ED50 of 20-100 ng/mL." class="big_lightbox" target="_blank">
The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to tyrosine phosphorylation of JAK1 and Tyk2, but not JAK2 or JAK3. In addition to its role as an antagonist of IFN-gamma-mediated macrophage activation, IL-10Ralpha transmits differentiation as well as proliferative signals. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.
![Neutralization IL-10R alpha Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-10_R_alpha_AF-474-NA_Block_Neutralize_8724.jpg)
![Binding Activity IL-10R alpha [Biotin]](http://images.novusbio.com/images/protein/avi9044_recombinant-human-il-10-r-alpha-fc-avi-tag-protein-cf-binding-activity-19112020154359.jpg)
![Inhibition Activity IL-10R alpha [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL10_R_alpha_10186RI_2632.jpg)
![Immunocytochemistry/ Immunofluorescence IL-10R alpha Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-474-na_mouse-il-10-r-alpha-affinity-purified-polyclonal-ab-8220249505988.jpg)
![Western Blot IL-10R alpha Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-474-na_mouse-il-10-r-alpha-affinity-purified-polyclonal-ab-8220249501921.jpg)
![Western Blot IL-10R alpha Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-474-na_mouse-il-10-r-alpha-affinity-purified-polyclonal-ab-8220249501918.jpg)
![Immunocytochemistry/ Immunofluorescence IL-10R alpha Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-474-na_mouse-il-10-r-alpha-affinity-purified-polyclonal-ab-8220249504613.jpg)
![N/A IL-10R alpha Antibody [Unconjugated]](http://images.novusbio.com/fullsize/antibody/af-474-na_mouse-il-10-r-alpha-affinity-purified-polyclonal-ab-822024102949..jpg)
![SDS-Page IL-10R alpha [Biotin]](http://images.novusbio.com/fullsize/protein/avi9044_recombinant-human-il-10-r-alpha-fc-avi-tag-protein-cf-sds-page-311202013590.jpg)
![SDS-Page IL-10R alpha [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL10_R_alpha_10186RI_2554.jpg)
