![Immunohistochemistry-Paraffin: IL-1 RII Antibody (RM0014-7D7) [NB110-85472] - IL1RII immunohistochemistry staining of paraffin sections of mouse Spleen and liver tissues from LPS exposed animals.](http://images.novusbio.com/fullsize2/IL-1-RII-Antibody-(RM0014-7D7)-Immunohistochemistry-Paraffin-NB110-85472-img0004.jpg "Immunohistochemistry-Paraffin: IL-1 RII Antibody (RM0014-7D7) [NB110-85472] - IL1RII immunohistochemistry staining of paraffin sections of mouse Spleen and liver tissues from LPS exposed animals.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, IHC, IHC-P |
| Clone | RM0014-7D7 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Mouse IL-1 RII (extracellular domain) |
| Specificity | This recognizes extracellular domain of mouse IL1R-II protein. |
| Isotype | IgG2 |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | IL1R2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Protein A or G purified |
| Reconstitution Instructions | Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml. |
![Dual RNAscope ISH-IHC: TLR4 Antibody (76B357.1) [NB100-56566] - FFPE tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, ACD catalog # 311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog # NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Dual-RNAscope-ISH-IHC-NB100-56566-img0036.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Immunofluorescent staining of TMAs. Representative tissue cores from normal (I), adenomatous polyps (II), and CRC (III and IV) are shown. Image collected and cropped by CiteAb from the following publication (http://www.jeccr.com/content/33/1/45), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Immunohistochemistry-NB100-56566-img0037.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (http://www.jeccr.com/content/33/1/45), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TLR4-Antibody-(76B357.1)-Immunohistochemistry-NB100-56566-img0040.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0014.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0015.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Protein-Array-NBP2-79843-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg)
![Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.](http://images.novusbio.com/images2/ZEB2-Antibody-Immunohistochemistry-Paraffin-NBP1-82991-img0014.jpg)
![Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human colon shows moderate nuclear positivity in a subset of lymphoid cells.](http://images.novusbio.com/images2/ZEB2-Antibody-Immunohistochemistry-Paraffin-NBP1-82991-img0015.jpg)
![Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human glioma shows moderate nuclear positivity in tumor cells.](http://images.novusbio.com/images2/ZEB2-Antibody-Immunohistochemistry-Paraffin-NBP1-82991-img0016.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for IL-1 RII Antibody (NB110-85472)Discover more about diseases related to IL-1 RII Antibody (NB110-85472).
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![Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by Goat anti-Rat IgG (H+L) Secondary Antibody [HRP]. Image from verified customer review.](http://images.novusbio.com/images2/Goat-anti-Rat-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7115-img0005.jpg "Western Blot: Goat anti-Rat IgG (H+L) Secondary Antibody [HRP] [NB7115] - Detection of DDK in SUSD3 overexpression lysate using anti-DYKDDDDK Epitope Tag antibody (cat.# NBP1-06712) followed by Goat anti-Rat IgG (H+L) Secondary Antibody [HRP]. Image from verified customer review.")