IL-1 RII Antibody (RM0014-7D7)

Immunohistochemistry-Paraffin: IL-1 RII Antibody (RM0014-7D7) [NB110-85472] - IL1RII immunohistochemistry staining of paraffin sections of mouse Spleen and liver tissues from LPS exposed animals.

• Reactivity: Mu
• Applications: WB, IHC, IHC-P
• Clone: RM0014-7D7
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 RII Antibody (RM0014-7D7) Summary

• Immunogen: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with mouse Recombinant extracellular domain of mouse IL1R-II protein.
• Specificity: This recognizes extracellular domain of mouse IL1R-II protein.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: IL1R2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-100

Reactivity Notes

This antibody is reactive against Mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.2 ml sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for IL-1 RII Antibody (RM0014-7D7)

• beta
• CD121b antigen
• CD121b
• IL-1 R beta
• IL-1 RII
• IL1R2
• IL1RBCD121 antigen-like family member B
• IL-1R-beta
• IL1RII
• IL-1RII
• IL-1RT2
• IL-1RT-2
• interleukin 1 receptor, type II
• Interleukin-1 receptor beta
• MGC47725

Background

IL-1 receptor type II (IL1R-II) is a 65 kD member of the immunoglobulin superfamily also known as IL-1 receptor (IL-1R) type II. The IL-1R family is composed of three proteins: IL-1R type I, IL-1R type II, and the widely expressed IL-1R AcP. Both IL-1R type I and type II bind IL-1 alpha and IL-1 beta, although with differing affinities. The high affinity complex consists of IL-1R type I and IL-1R AcP, whereas IL-1 binds to IL-1R type II with low affinity. The IL-1 receptor antagonist (IL-1RA) can bind to both IL-1R type I and II. IL-1R type II is expressed at low levels on B cells, monocytes/macrophages, neutrophils, basal epithelia of the skin, and T cells. Binding of IL-1 to the high affinity IL-1 receptor mediates thymocyte and T cell activation, fibroblast proliferation, and induction of acute phase protein production. Binding of IL-1 to CDw121b appears to be dispensable for IL-1 signaling and may negatively regulate IL-1 responses acting as a "decoy" receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL1R2
• Entrez:
  • 16178(Mouse)