IL-1 beta/IL-1F2 Antibody [Unconjugated]

Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑1 beta /IL‑1F2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681). The ND50 is typically 4‑20 µg/mL.

Silencing of TREM2 increases the production of inflammatory cytokines and type I interferons.(A) PAMs were transfected with siNC or siTREM2-1 for 24 h, gene expression of IL-1 beta , IL-6, IL-8, TNF-alpha , IFN-alpha , and IFN-beta are shown using qRT-PCR. (B) Protein expression of IL-1 beta , IL-10, IL-4, and TNF-alpha are shown, as detected by western blot analysis in the TREM2 knockdown assay. GAPDH is shown as an internal control. (C) PAMs were transfected with siNC or siTREM2-1 for 24 h and infected with PPRSV (MOI = 1). Cells were collected at the indicated periods. Proinflammatory cytokine (IL-1 beta , IL-6, and IL-8) and type I interferons (IFN-alpha and IFN-beta ) mRNA expression is shown, as detected by qRT-PCR. (D) PAMs were treated as described in A and infected with PRRSV at different MOIs (0.5 or 1), transcription of IL-1 beta , TNF-alpha , and IFN-beta is shown, as measured by qRT-PCR. Data are representative of the results of three independent experiments (mean ± SE). Significant differences compared to the control group are denoted by * (P <.05), ** (P <.01) and *** (P <.001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32401783), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Po
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant porcine IL‑1 beta /IL‑1F2
  Ala115-Pro267
  Accession # P26889
• Specificity: Detects porcine IL‑1 beta /IL‑1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45%‑75% cross‑reactivity with recombinant human IL‑1 beta , recombinant mouse IL‑1 beta , recombinant rat IL‑1 beta , recombinant feline IL‑1 beta , recombinant canine IL‑1 beta , recombinant equine IL‑1 beta , recombinant guinea pig IL‑1 beta , recombinant rabbit IL‑1 beta , and recombinant rhesus monkey IL‑1 beta is observed and less than 15% cross-reactivity with recombinant cotton rat IL-1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1B
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 4-20 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63%-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta .

1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
5. Huether, M. et al. (1993) Gene 129:285.
6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (AF681)(6)

Publications using AF681

• Kiczak, L;Pasławska, U;Goździk, W;Adamik, B;Zielińska, M;Zieliński, S;Nowak, K;Płóciennik, M;Bania, J;Tabiś, A;Nowak, M;Pasławski, R;Frostell, C; Effect of low-dose hydrocortisone and inhaled nitric oxide on inflammatory mediators and local pulmonary metalloproteinases activity in LPS-induced sepsis in piglets Scientific reports 2023-07-13 [PMID: 37443327] (Western Blot, Porcine)
• Sheng He, Lu Li, Haifan Chen, Xiaoli Hu, Wendi Wang, Hui Zhang et al. PRRSV Infection Induces Gasdermin D-Driven Pyroptosis of Porcine Alveolar Macrophages through NLRP3 Inflammasome Activation Journal of Virology 2022-07-27 [PMID: 35758658]
• Z Zhu, X Zhang, W Dong, X Wang, S He, H Zhang, X Wang, R Wei, Y Chen, X Liu, C Guo TREM2 suppresses the proinflammatory response to facilitate PRRSV infection via PI3K/NF-kappaB signaling PLoS Pathog., 2020-05-13;16(5):e1008543. 2020-05-13 [PMID: 32401783] (Co-Immunoprecipitation, ICC, Western Blot, Porcine)
• HS Park, G Liu, SN Thulasi Ra, SL Landreth, Q Liu, Y Zhou NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination J. Virol., 2018-03-28;0(0):. 2018-03-28 [PMID: 29386291] (Western Blot, Porcine)
• Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts. Eur. Heart J., 2008-05-02;29(12):1578-90. 2008-05-02 [PMID: 18456710] (Western Blot, Porcine)
• Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J Induction of interleukin-1 in articular cartilage by explantation and cutting. Arthritis Rheum., 2004-08-01;50(8):2539-46. 2004-08-01 [PMID: 15334468] (Neutralization, Porcine)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P26889()