IL-1 beta/IL-1F2 Antibody [Unconjugated]

Recombinant Feline IL‑1 beta /IL‑1F2 (Catalog # 1796‑FL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796). The ND50 is typically 0.02‑0.08 µg/mL in the presence of concanavalin A (1.25 µg/mL).

• Reactivity: Fe
• Applications: WB, B/N, ELISA(Cap), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant feline IL‑1 beta /IL‑1F2
  Ala116-Ser267
  Accession # P41687
• Specificity: Detects IL‑1 beta /IL‑1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human IL‑1 beta, recombinant mouse IL-1 beta, and recombinant canine IL-1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1B
• Purity: Immunogen affinity purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.02-0.08 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: Standard:Recombinant Feline IL-1 beta/IL-1F2 (Catalog # 1796-FL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in feline. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with the IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The feline IL-1 beta cDNA encodes a 267 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature feline IL-1 beta shares 63%‑78% aa sequence identity with canine, cotton rat, equine, human, mouse, porcine, rat, and rhesus IL-1 beta.

1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
5. Accession # AAA30814.
6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IL-1 beta/IL-1F2 Antibody (AF1796) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Feline.

reviewed by: Anonymous IHC-Fr Feline 11/16/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: brain and spinal cord
• Species: Feline
• Lot: KAM011809A

Comments

• Comments: AF1796 antibody was used at 1:200 dilution.

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • P41687()