IL-1 beta/IL-1F2 Antibody [Unconjugated]

IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑1 beta /IL‑1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND50 is typically 2-10 µg/mL.

• Reactivity: Rt
• Applications: WB, B/N, ELISA(Cap), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat IL-1 beta /IL-1F2 (R&D Systems, Catalog # 501-RL)
  Val117-Ser268
  Accession # Q63264
• Specificity: Detects rat IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL‑1 beta is observed and less than 0.5% cross-reactivity with recombinant human IL-1 beta and recombinant porcine IL-1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1B
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Rat IL-1 beta/IL-1F2 Antibody (Catalog # AF-501-NA)
  ELISA Detection: Rat IL-1 beta/IL-1F2 Biotinylated Antibody (Catalog # BAF501)
  Standard: Recombinant Rat IL-1 beta/IL-1F2 (Catalog # 501-RL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody [Unconjugated]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (AF-501-NA)(37)

Publications using AF-501-NA

• MDM Fernández-, JM Grondona, P Fernández-, MD López-Ával Microglial activation by microbial neuraminidase through TLR2 and TLR4 receptors J Neuroinflammation, 2019;16(1):245. 2019 [PMID: 31791382] (IHC, Mouse)
• M Yamamoto, M Kim, H Imai, Y Itakura, G Ohtsuki Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation Cell Rep, 2019;28(11):2923-2938.e8. 2019 [PMID: 31509752] (Western Blot, Rat)
• ZH Wang, J Xiang, X Liu, SP Yu, FP Manfredsso, IM Sandoval, S Wu, JZ Wang, K Ye Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBP? in Alzheimer&#039;s Disease Cell Rep, 2019;28(3):655-669.e5. 2019 [PMID: 31315045] (Neutralization, Rat)
• HC Lai, CH Lu, CS Wong, BF Lin, SM Chan, CY Kuo, ZF Wu Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection J Chin Med Assoc, 2018;0(0):. 2018 [PMID: 30139671] (Rat)
• CH Jo, S Kim, JS Park, GH Kim Anti-Inflammatory Action of Sitagliptin and Linagliptin in Doxorubicin Nephropathy Kidney Blood Press. Res., 2018;43(3):987-999. 2018 [PMID: 29913457] (ELISA Development (Capture), Rat)
• P Zhang, RY Bi, YH Gan Glial interleukin-1? upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats J Neuroinflammation, 2018;15(1):117. 2018 [PMID: 29678208] (Western Blot, Rat)
• L Taoro-Gonz, YM Arenas, A Cabrera-Pa, V Felipo Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms J Neuroinflammation, 2018;15(1):36. 2018 [PMID: 29422059] (Western Blot, Rat)
• HC Wang, HS Tsay, HN Shih, YA Chen, KM Chang, DC Agrawal, S Huang, YL Lin, MJ Lee Andrographolide relieved pathological pain generated by spared nerve injury model in mice Pharm Biol, 2018;56(1):124-131. 2018 [PMID: 29385888] (IHC, Mouse)
• CC Shih, HR Hwang, CI Chang, HM Su, PC Chen, HM Kuo, PJ Li, HD Wang, KH Tsui, YC Lin, SY Huang, ZH Wen Anti-Inflammatory and Antinociceptive Effects of Ethyl Acetate Fraction of an Edible Red Macroalgae Sarcodia ceylanica Int J Mol Sci, 2017;18(11):. 2017 [PMID: 29149031] (IHC, Rat)
• R Natoli, N Fernando, M Madigan, JA Chu-Tan, K Valter, J Provis, M Rutar Microglia-derived IL-1? promotes chemokine expression by M�ller cells and RPE in focal retinal degeneration Mol Neurodegener, 2017;12(1):31. 2017 [PMID: 28438165] (Neutralization, Rat)
• Congli Yuan Inhibition of phagocytosis and pyroptosis of macrophages promotes Bartonella invasion into the bloodstream through lymphatic circulation J. Infect. Dis., 2016;0(0):. 2016 [PMID: 27803173] (Western Blot, Rat)
• In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues Molecules, 2016;21(8):. 2016 [PMID: 27483221] (ELISA Development, Mouse)
• V Hernandez-, A Cabrera-Pa, L Taoro-Gonz, A Gonzalez-U, A Agusti, T Balzano, M Llansola, V Felipo Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia J Neuroinflammation, 2016;13(1):83. 2016 [PMID: 27090509]
• Jiao H, Natoli R, Valter K, Provis J, Rutar M Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration. PLoS ONE, 2015;10(12):e0143952. 2015 [PMID: 26630454] (IHC-Fr, Rat)
• Madeira M, Elvas F, Boia R, Goncalves F, Cunha R, Ambrosio A, Santiago A Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure. J Neuroinflammation, 2015;12(0):115. 2015 [PMID: 26054642] (Neutralization, Rat)
• Silva G, Lopes P, Fonoff E, Pagano R The spinal anti-inflammatory mechanism of motor cortex stimulation: cause of success and refractoriness in neuropathic pain? J Neuroinflammation, 2015;12(0):10. 2015 [PMID: 25600429] (IHC, Rat)
• Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, Molina-Holgado F, Garcia-Ovejero D Spinal cord injury induces a long-lasting upregulation of interleukin-1beta in astrocytes around the central canal. Neuroscience, 2015;284(0):283-9. 2015 [PMID: 25453765] (IHC, Rat)
• Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang T Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats. PLoS ONE, 2014;9(8):e106331. 2014 [PMID: 25170959] (IHC-Fr, Rat)
• Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone. J Neurosci, 2014;34(6):2231-43. 2014 [PMID: 24501362] (Neutralization, Rat)
• Liu R, Gao J, Cao S, Sandhu N, Cui J, Chou C, Fang E, Matsubara J Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration. Invest Ophthalmol Vis Sci, 2013;54(3):2225-37. 2013 [PMID: 23462752] (IHC-P, Rat)
• Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain. J Neuroinflammation, 2012;9(0):181. 2012 [PMID: 22824437] (IHC-Fr, Rat)
• Faustino JV, Wang X, Johnson CE Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke. J. Neurosci., 2011;31(36):12992-3001. 2011 [PMID: 21900578] (IHC, Rat)
• Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes. J. Neurochem., 2011;117(1):164-74. 2011 [PMID: 21244430] (Western Blot, Rat)
• Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia. J. Neurochem., 2011;116(2):217-26. 2011 [PMID: 21054390] (IHC-Fr, Rat)
• Summy-Long JY, Hu S Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus. Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009;297(5):R1532-45. 2009 [PMID: 19759333] (IHC, Rat)
• Lim G, Wang S, Zhang Y, Tian Y, Mao J Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents. J. Clin. Invest., 2009;119(2):295-304. 2009 [PMID: 19139561] (Western Blot, Rat)
• Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology. Infect. Immun., 2008;76(8):3577-86. 2008 [PMID: 18541654] (ELISA Development, Rat)
• Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland. Endocrinology, 2008;149(4):1436-50. 2008 [PMID: 18174279] (IHC-Fr, Rat)
• Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway. Biochem. Biophys. Res. Commun., 2007;365(2):375-80. 2007 [PMID: 17996195] (ICC, Rat)
• Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor. Am. J. Physiol. Heart Circ. Physiol., 2007;293(3):H1839-46. 2007 [PMID: 17616748] (Neutralization, Rat)
• Roche M, Diamond M, Finn DP In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids. J. Neuroimmunol., 2006;181(1):57-67. 2006 [PMID: 17011047] (ELISA Development, Rat)
• Barkho BZ, Song H, Aimone JB Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 2006;15(3):407-21. 2006 [PMID: 16846377] (Neutralization, Rat)
• Assmus M, Svechnikov K, von Euler M, Setchell B, Sultana T, Zetterstrom C, Holst M, Soder O Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines. Pediatr. Res., 2005;57(6):896-901. 2005 [PMID: 15845639] (IHC, Rat)
• Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression. Neuroscience, 2005;132(3):625-31. 2005 [PMID: 15837124] (Western Blot, Rat)
• Guo L, LaDu MJ, Van Eldik LJ A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity. J. Mol. Neurosci., 2004;23(3):205-12. 2004 [PMID: 15181248] (Western Blot, Rat)
• Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats. J. Physiol. (Lond.), 2004;556(0):193-202. 2004 [PMID: 14724215] (IHC-Fr, Rat)
• Nadeau S, Rivest S Glucocorticoids play a fundamental role in protecting the brain during innate immune response. J. Neurosci., 2003;23(13):5536-44. 2003 [PMID: 12843254] (Neutralization, Rat)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • Q63264()