Recombinant Rat IL-1 alpha/IL-1F1 Protein

SDS-Page: Rat IL-1 alpha/IL-1F1 Protein [NBP2-35227]

• Reactivity: Rt
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat IL-1 alpha/IL-1F1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 156 amino acids corresponding to IL-1 alpha/IL-1F1 Source: E. coli

  Uniprot ID: P16598

  Amino Acid Sequence: SAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDDSKYP VTLKVSNTQL FVSAQGEDKP VLLKEIPETP KLITGSETDL IFFWEKINSK NYFTSAAFPE LLIATKEQSQ VHLARGLPSM IDFQIS

• Details of Functionality: IL1 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.005 ng/ml, corresponding to a specific activity of > 2.0 x 10^8 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL1A
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-1 alpha/IL-1F1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat IL-1 alpha/IL-1F1 Protein

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1alpha) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1alpha and IL-1beta binds to the same receptor and has similar but not identical biological properties. Recombinant rat IL-1 alpha contains 150 amino acids which is a single non-glycosylated polypeptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for IL-1 alpha/IL-1F1 Recombinant Protein (NBP2-35227)(3)

Publications using NBP2-35227

• Krista A Shisler, William M Kincannon, Jenna R Mattice, James Larson, Adam Valaydon-Pillay, Florence Mus, Tamara Flusche, Arnab Kumar Nath, Sebastian A Stoian, Simone Raugei, Brian Bothner, Jennifer L DuBois, John W Peters Homologous acetone carboxylases select Fe(II) or Mn(II) as the catalytic cofactor. mBio 2023-12-21 [PMID: 38126751]
• Luping Chen, Ling Ding, Xian Wang, Yufei Huang, Shou-Jiang Gao, Shou-Wei Ding Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesis mBio 2024-01-01 [PMID: 38117084]
• Chen L, Ding L, Wang X et al. Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesis bioRxiv : the preprint server for biology 2023-11-14 [PMID: 38014281]

Bioinformatics

• Gene Symbol: IL1A
• Uniprot:
  • P16598()
  • P16598()
  • P16598()