Recombinant Rat IL-1 alpha/IL-1F1 Protein

Images

 
SDS-Page: Rat IL-1 alpha/IL-1F1 Protein [NBP2-35227]

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Rat IL-1 alpha/IL-1F1 Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 156 residues of IL1A.

Source: E. coli

Amino Acid Sequence: SAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDDSKYP VTLKVSNTQL FVSAQGEDKP VLLKEIPETP KLITGSETDL IFFWEKINSK NYFTSAAFPE LLIATKEQSQ VHLARGLPSM IDFQIS

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
IL1 alpha Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.005 ng/ml, corresponding to a specific activity of > 2.0 x 10^8 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IL1A
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
17.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5.
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Rat IL-1 alpha/IL-1F1 Protein

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Interleukin-1 alpha (IL-1alpha) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1alpha and IL-1beta binds to the same receptor and has similar but not identical biological properties. Recombinant rat IL-1 alpha contains 150 amino acids which is a single non-glycosylated polypeptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

7954-GM/CF
Species: Hu
7954-GM/CF
Species: Hu
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
796-IC
Species: Mu
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NBP2-35227
Species: Rt
Applications: Func, PAGE, Bioactivity

Publications for IL-1 alpha/IL-1F1 Protein (NBP2-35227) (0)

There are no publications for IL-1 alpha/IL-1F1 Protein (NBP2-35227).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-1 alpha/IL-1F1 Protein (NBP2-35227) (0)

There are no reviews for IL-1 alpha/IL-1F1 Protein (NBP2-35227). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for IL-1 alpha/IL-1F1 Protein (NBP2-35227) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for IL-1 alpha/IL-1F1 Protein (NBP2-35227)

Discover related pathways, diseases and genes to IL-1 alpha/IL-1F1 Protein (NBP2-35227). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-1 alpha/IL-1F1 Protein (NBP2-35227)

Discover more about diseases related to IL-1 alpha/IL-1F1 Protein (NBP2-35227).
 

Pathways for IL-1 alpha/IL-1F1 Protein (NBP2-35227)

View related products by pathway.

PTMs for IL-1 alpha/IL-1F1 Protein (NBP2-35227)

Learn more about PTMs related to IL-1 alpha/IL-1F1 Protein (NBP2-35227).
 

Research Areas for IL-1 alpha/IL-1F1 Protein (NBP2-35227)

Find related products by research area.

Blogs on IL-1 alpha/IL-1F1

There are no specific blogs for IL-1 alpha/IL-1F1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4
6507-IL-010/CF
IL-1 alpha/IL-1F1
200-LA-002/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Rat IL-1 alpha/IL-1F1 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol IL1A
Uniprot