Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

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Human IL-1 alpha/IL-1F1 ELISA Kit [NBP1-91187] - Data for Human IL-1alpha Platinum ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
1.6 - 100 pg/mL
Sensitivity
1.1 pg/mL

Order Details

Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-1 alpha positive serum. There was no crossreactivity detected.
Standard Curve Range
1.6 - 100 pg/mL
Sensitivity
1.1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
10%
Intra-Assay
5.40%
Spike Recovery
The amount of endogenous human IL-1 alpha in unspiked samples was subtracted from the spike values. The overall mean recovery was 92%.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL1A

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Substrate Solution (tetramethyl-benzidine)
  2. Streptavidin-HRP
  3. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  4. Stop Solution (1M Phosphoric acid)
  5. Sample Diluent
  6. Red-Dye
  7. Blue-Dye
  8. Green-Dye
  9. Human IL-1alpha Standard lyophilized, 200 pg/mL upon reconstitution
  10. Adhesive Films
  11. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human IL-1alpha
  12. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  13. Biotin-Conjugate anti-human IL-1alpha polyclonal antibody

Alternate Names for Human IL-1 alpha/IL-1F1 ELISA Kit (Colorimetric)

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct human IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation. IL-1alpha is an extracellular peptide of 17 kDa, its activity has been demonstrated in various biological fluids including serum, synovial fluid, gingival fluid, amniotic fluid, sputum, cerebrosinal fluid, urine, and bronchoalveolar lavage (BAL) fluid. Elevated serum or blood levels of IL-1alpha have been found in patients with total hip replacement/ arthroplasties, in patients with recently diagnosted IDDM (Insulin-Dependent Diabetes Mellitus), in case of several carcinomas such as head and neck cancer, pancreatic cancer and thyroid cancer, in experimental acute pyelonephritis, in acute viral hepatitis and in septic shock.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL1A
Entrez