Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves th...at were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.
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• Specificity: Natural and recombinant human IL-1 alpha
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL1A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-1 alpha/IL-1F1 (DLA50)(53)

Publications using DLA50

• Koutaki, D;Paltoglou, G;Manou, M;Vourdoumpa, A;Ramouzi, E;Tzounakou, AM;Michos, A;Bacopoulou, F;Mantzou, E;Zoumakis, E;Papadopoulou, M;Kassari, P;Charmandari, E; The Role of Secreted Frizzled-Related Protein 5 (Sfrp5) in Overweight and Obesity in Childhood and Adolescence Nutrients 2024-09-17 [PMID: 39339733] (Human)
• Kutuk, MO;Tufan, AE;Kilicaslan, F;Gokcen, C;Aksu, GG;Yektas, C;Kandemir, H;Celik, F;Mutluer, T;Buber, A;Karadag, M;Coban, N;Coskun, S;Hangul, Z;Altintas, E;Acikbas, U;Giray, A;Aka, Y;Basturk, B;Kutuk, O; Cytokine expression profiles in children and adolescents with tic disorders Scientific reports 2024-07-02 [PMID: 38956051] (Human)
• Azumi, Y;Koma, YI;Tsukamoto, S;Kitamura, Y;Ishihara, N;Yamanaka, K;Nakanishi, T;Miyako, S;Urakami, S;Tanigawa, K;Kodama, T;Nishio, M;Shigeoka, M;Kakeji, Y;Yokozaki, H; IFI16 Induced by Direct Interaction between Esophageal Squamous Cell Carcinomas and Macrophages Promotes Tumor Progression via Secretion of IL-1? Cells 2023-11-10 [PMID: 37998338] (Human)
• Jung, SH;Chung, KS;Na, CS;Ahn, HS;Shin, YK;Lee, KT; Ethanol Extracts from the Aerial Parts of Inula japonica and Potentilla chinensis Alleviate Airway Inflammation in Mice That Inhaled Particulate Matter 10 and Diesel Particulate Matter Nutrients 2023-10-29 [PMID: 37960252] (Mouse)
• Egan, MS;O'Rourke, EA;Mageswaran, SK;Zuo, B;Martynyuk, I;Demissie, T;Hunter, EN;Bass, AR;Chang, YW;Brodsky, IE;Shin, S; Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages bioRxiv : the preprint server for biology 2023-07-18 [PMID: 37503120] (Human)
• S Kang, H Lee, SH Jun, SG Park, NG Kang Enhancement of Efficacy of Retinoids through Enhancing Retinoid-Induced RAR Activity and Inhibiting Hydroxylation of Retinoic Acid, and Its Clinical Efficacy on Photo-Aging Pharmaceutics, 2022-11-08;14(11):. 2022-11-08 [PMID: 36365229] (Human)
• P Szczepanik, M Michalska-, M Kowal, D Krasowska Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea Journal of Clinical Medicine, 2022-10-28;11(21):. 2022-10-28 [PMID: 36362603] (Human)
• ZQ Zhang, ZP Guo, XY Lv, P Sörös, XX Wang, L Wang, CH Liu Effect and neural mechanisms of the transcutaneous vagus nerve stimulation for relapse prevention in patients with remitted major depressive disorder: protocol for a longitudinal study BMJ Open, 2022-02-22;12(2):e050446. 2022-02-22 [PMID: 35193903] (Human)
• P Montero, J Milara, M Pérez-Leal, C Estornut, I Roger, A Pérez-Fida, C Sanz, J Cortijo Paclitaxel-Induced Epidermal Alterations: An In Vitro Preclinical Assessment in Primary Keratinocytes and in a 3D Epidermis Model International Journal of Molecular Sciences, 2022-01-20;23(3):. 2022-01-20 [PMID: 35163066] (Human)
• F Rancan, X Guo, K Rajes, P Sidiropoul, F Zabihi, L Hoffmann, S Hadam, U Blume-Peyt, E Rühl, R Haag, A Vogt Topical Delivery of Rapamycin by Means of Microenvironment-Sensitive Core-Multi-Shell Nanocarriers: Assessment of Anti-Inflammatory Activity in an ex vivo Skin/T Cell Co-Culture Model International Journal of Nanomedicine, 2021-10-22;16(0):7137-7151. 2021-10-22 [PMID: 34712046] (Human)
• B Sikora, A Skubis-Sik, A Prusek, J Gola Paracrine activity of adipose derived stem cells on limbal epithelial stem cells Scientific Reports, 2021-10-07;11(1):19956. 2021-10-07 [PMID: 34620960] (Human)
• U Zlabiene, J Baranauska, DM Kopustinsk, J Bernatonie In Vitro and Clinical Safety Assessment of the Multiple W/O/W Emulsion Based on the Active Ingredients from Rosmarinus officinalis L., Avena sativa L. and Linum usitatissimum L Pharmaceutics, 2021-05-16;13(5):. 2021-05-16 [PMID: 34065623] (Human)
• L Avila, ML Neves, AR Abreu, CR Fiuza, L Fukusawa, N Meziat-Fil, AR Soares San Cognitive functional therapy (CFT) compared with core training exercise (CTE) in patients with failed back surgery syndrome (FBSS): A study protocol for a randomized controlled trial Journal of bodywork and movement therapies, 2020-09-14;26(0):428-434. 2020-09-14 [PMID: 33992278] (Human)
• H Yang, X Yang, Y Wang, X Zheng, Y Zhang, Y Shao Comparative analysis of the tear protein profile in herpes simplex virus type 1 epithelial keratitis BMC Ophthalmol, 2020-08-31;20(1):355. 2020-08-31 [PMID: 32867704] (Human)
• MO Kutuk, E Tufan, C Gokcen, F Kilicaslan, M Karadag, T Mutluer, C Yektas, N Coban, H Kandemir, A Buber, S Coskun, U Acikbas, G Guler, Z Topal, F Celik, E Altintas, A Giray, Y Aka, O Kutuk Cytokine expression profiles in Autism spectrum disorder: A multi-center study from Turkey Cytokine, 2020-06-18;133(0):155152. 2020-06-18 [PMID: 32563959] (Human)
• P Anantawora, W Chaiyana, BB Michniak-K, W Rungseevij, C Ampasavate Enhanced Transdermal Delivery of Concentrated Capsaicin from Chili Extract-Loaded Lipid Nanoparticles with Reduced Skin Irritation Pharmaceutics, 2020-05-19;12(5):. 2020-05-19 [PMID: 32438539] (Human)
• K Babiuch, B Ku?nierz-C, B K?sek, K Oko?, D Darczuk, M Chomyszyn- Evaluation of Proinflammatory, NF-kappaB Dependent Cytokines: IL-1&alpha, IL-6, IL-8, and TNF-&alpha in Tissue Specimens and Saliva of Patients with Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders J Clin Med, 2020-03-21;9(3):. 2020-03-21 [PMID: 32245251] (Human)
• M Pein, J Insua-Rodr, T Hongu, A Riedel, J Meier, L Wiedmann, K Decker, MAG Essers, HP Sinn, S Spaich, M Sütterlin, A Schneeweis, A Trumpp, T Oskarsson Metastasis-initiating cells induce and exploit a fibroblast niche to fuel malignant colonization of the lungs Nat Commun, 2020-03-20;11(1):1494. 2020-03-20 [PMID: 32198421] (Human)
• M Sugiyama, T Ueki, S Ogata, H Itagaki Predicting the results of a 24-hr human patch test for surfactants: utility of margin-setting in a reconstructed human epidermis model J Toxicol Sci, 2019-01-01;44(6):393-403. 2019-01-01 [PMID: 31167989] (Human)
• Y Tanaka, H Uchi, A Hashimoto-, M Furue Tryptophan Photoproduct FICZ Upregulates IL1A, IL1B, and IL6 Expression via Oxidative Stress in Keratinocytes Oxid Med Cell Longev, 2018-11-25;2018(0):9298052. 2018-11-25 [PMID: 30595799] (Human)
• J Hooda, M Novak, MP Salomon, C Matsuba, RI Ramos, E MacDuffie, M Song, MS Hirsch, J Lester, V Parkash, BY Karlan, M Oren, DS Hoon, R Drapkin Early loss of Histone H2B monoubiquitylation alters chromatin accessibility and activates key immune pathways that facilitate progression of ovarian cancer Cancer Res., 2018-12-18;0(0):. 2018-12-18 [PMID: 30563893] (Human)
• AF Siqueira, A Vieira, M Bottaro, JB Ferreira-J, OT Nóbrega, VC de Souza, RC Marqueti, N Babault, JLQ Durigan Multiple Cold-Water Immersions Attenuate Muscle Damage but not Alter Systemic Inflammation and Muscle Function Recovery: A Parallel Randomized Controlled Trial Sci Rep, 2018-07-19;8(1):10961. 2018-07-19 [PMID: 30026562] (Human)
• C Wyatt Shie, JP White, EG Osta, J Patel, S Rajkumar, N Kirby, JP Therrien, S Zauscher Encapsulation and controlled release of retinol from silicone particles for topical delivery J Control Release, 2018-03-28;0(0):. 2018-03-28 [PMID: 29604311] (Human)
• A Scandolera, J Hubert, A Humeau, C Lambert, A De Bizemon, C Winkel, A Kaouas, JH Renault, JM Nuzillard, R Reynaud GABA and GABA-Alanine from the Red Microalgae Rhodosorus marinus Exhibit a Significant Neuro-Soothing Activity through Inhibition of Neuro-Inflammation Mediators and Positive Regulation of TRPV1-Related Skin Sensitization Mar Drugs, 2018-03-17;16(3):. 2018-03-17 [PMID: 29562624] (Human)
• Y Zhou, M Liao, C Zhu, Y Hu, T Tong, X Peng, M Li, M Feng, L Cheng, B Ren, X Zhou ERG3 and ERG11 genes are critical for the pathogenesis of Candida albicans during the oral mucosal infection Int J Oral Sci, 2018-03-16;10(2):9. 2018-03-16 [PMID: 29555898] (Human)
• Y Yang, S Lian, L Meng, L Qu, C Shou Antibody Array Revealed PRL-3 Affects Protein Phosphorylation and Cytokine Secretion PLoS ONE, 2017-01-09;12(1):e0169665. 2017-01-09 [PMID: 28068414] (Human)
• Tatsuya Hasegawa Nuclear DNA damage-triggered NLRP3 inflammasome activation promotes UVB-induced inflammatory responses in human keratinocytes Biochem Biophys Res Commun, 2016-06-23;0(0):. 2016-06-23 [PMID: 27343554] (Human)
• Koch A, Love-Homan L, Espinosa-Cotton M, Stanam A, Simons A MyD88-Dependent Signaling Decreases the Antitumor Efficacy of Epidermal Growth Factor Receptor Inhibition in Head and Neck Cancer Cells. Cancer Res, 2015-02-20;75(8):1657-67. 2015-02-20 [PMID: 25712126] (Human)
• Horbelt D, Boergermann J, Chaikuad A, Alfano I, Williams E, Lukonin I, Timmel T, Bullock A, Knaus P Small molecules dorsomorphin and LDN-193189 inhibit myostatin/GDF8 signaling and promote functional myoblast differentiation. J Biol Chem, 2014-11-03;290(6):3390-404. 2014-11-03 [PMID: 25368322] (Human)
• Vambutas A, Lesser M, Mullooly V, Pathak S, Zahtz G, Rosen L, Goldofsky E Early efficacy trial of anakinra in corticosteroid-resistant autoimmune inner ear disease. J Clin Invest, 2014-08-18;124(9):4115-22. 2014-08-18 [PMID: 25133431] (Human)
• Pauwels NS, Bracke KR, Dupont LL, Van Pottelberge GR, Provoost S, Vanden Berghe T, Vandenabeele P, Lambrecht BN, Joos GF, Brusselle GG Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD. Eur. Respir. J., 2011-05-26;38(5):1019-28. 2011-05-26 [PMID: 21622588] (Human)
• Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects. Cancer Res., 2009-09-29;69(20):8191-9. 2009-09-29 [PMID: 19789336] (Human)
• Lachmann HJ, Lowe P, Felix SD, Rordorf C, Leslie K, Madhoo S, Wittkowski H, Bek S, Hartmann N, Bosset S, Hawkins PN, Jung T In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes. J. Exp. Med., 2009-04-13;206(5):1029-36. 2009-04-13 [PMID: 19364880] (Human)
• Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J. Exp. Med., 2009-01-12;206(1):169-81. 2009-01-12 [PMID: 19139172] (Human)
• Gouin JP, Kiecolt-Glaser JK, Malarkey WB, Glaser R The influence of anger expression on wound healing. Brain Behav. Immun., 2007-12-19;22(5):699-708. 2007-12-19 [PMID: 18078737] (Human)
• Scamuffa N, Siegfried G, Bontemps Y, Ma L, Basak A, Cherel G, Calvo F, Seidah NG, Khatib AM Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells. J. Clin. Invest., 2008-01-01;118(1):352-63. 2008-01-01 [PMID: 18064302] (Human)
• Niki Y, Takaishi H, Takito J, Miyamoto T, Kosaki N, Matsumoto H, Toyama Y, Tada N Administration of cyclooxygenase-2 inhibitor reduces joint inflammation but exacerbates osteopenia in IL-1 alpha transgenic mice due to GM-CSF overproduction. J. Immunol., 2007-07-01;179(1):639-46. 2007-07-01 [PMID: 17579086] (Human)
• Ishida T, Sakaguchi I Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon. Biol. Pharm. Bull., 2007-05-01;30(5):928-34. 2007-05-01 [PMID: 17473437] (Human)
• Arnold R, Neumann M, Konig W Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. Immunology, 2007-05-01;121(1):71-81. 2007-05-01 [PMID: 17425601] (Human)
• Zhu S, Su G, Meng QH Inhibitory effects of micronized fenofibrate on carotid atherosclerosis in patients with essential hypertension. Clin. Chem., 2006-09-21;52(11):2036-42. 2006-09-21 [PMID: 16990410] (Human)
• Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK The response of human epithelial cells to TNF involves an inducible autocrine cascade. Cell, 2006-03-24;124(6):1225-39. 2006-03-24 [PMID: 16564013] (Human)
• Fichorova RN Guiding the vaginal microbicide trials with biomarkers of inflammation. J. Acquir. Immune Defic. Syndr., 2004-10-01;37(0):S184-93. 2004-10-01 [PMID: 16419271] (Human)
• Dardik A, Yamashita A, Aziz F, Asada H, Sumpio BE Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1alpha. J. Vasc. Surg., 2005-02-01;41(2):321-31. 2005-02-01 [PMID: 15768016] (Bovine)
• Pagura SM, Thomas SG, Woodhouse LJ, Ezzat S, Marks P Circulating and synovial levels of IGF-I, cytokines, physical function and anthropometry differ in women awaiting total knee arthroplasty when compared to men. J. Orthop. Res., 2005-03-01;23(2):397-405. 2005-03-01 [PMID: 15734254] (Human)
• Yoshinari N, Kawase H, Mitani A, Ito M, Sugiishi S, Matsuoka M, Shirozu N, Ishihara Y, Bito B, Hiraga M, Arakawa K, Noguchi T Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues. J. Periodont. Res., 2004-06-01;39(3):158-67. 2004-06-01 [PMID: 15102044] (Human)
• Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action. Immunity, 2003-01-01;18(1):87-96. 2003-01-01 [PMID: 12530978] (Human)
• de Revel T, Becard N, Sorg T, Rousseau S, Spano JP, Thiebot H, Methali M, Gras G, Le Grand R, Dormont D Retroviral interleukin 1alpha gene transfer in bone marrow stromal cells in a primate model: induction of myelopoiesis stimulation. Br. J. Haematol., 2002-09-01;118(3):875-84. 2002-09-01 [PMID: 12181061] (Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Black , K P, Merrill , K W, Jackson , S, Katz , J Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity. Oral Microbiol Immunol, 2000-04-01;15(2):74-81. 2000-04-01 [PMID: 11155169] (Human)
• Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, Fusenig NE, Angel P c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cell, 2000-11-22;103(5):745-55. 2000-11-22 [PMID: 11114331] (Human)
• Kadowaki N, Antonenko S, Lau JY, Liu YJ Natural interferon alpha/beta-producing cells link innate and adaptive immunity. J. Exp. Med., 2000-07-17;192(2):219-26. 2000-07-17 [PMID: 10899908] (Human)
• Petitto JM, Leserman J, Perkins DO, Stern RA, Silva SG, Gettes D, Zheng B, Folds JD, Golden RN, Evans DL High versus low basal cortisol secretion in asymptomatic, medication-free HIV-infected men: differential effects of severe life stress on parameters of immune status. Behav Med, 2000-01-01;25(4):143-51. 2000-01-01 [PMID: 10789020] (Human)
• Rasmussen L, Hanstrom L, Lerner UH Characterization of bone resorbing activity in gingival crevicular fluid from patients with periodontitis. J. Clin. Periodontol., 2000-01-01;27(1):41-52. 2000-01-01 [PMID: 10674961] (Human)
• Lerner UH, Modeer T, Krekmanova L, Claesson R, Rasmussen L Gingival crevicular fluid from patients with periodontitis contains bone resorbing activity. Eur. J. Oral Sci., 1998-06-01;106(3):778-87. 1998-06-01 [PMID: 9672100] (Human)

Bioinformatics

• Gene Symbol: IL1A