IL-1 alpha/IL-1F1 Antibody [Unconjugated]

Images

 
Western blot shows 50 ng of Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400‑ML), Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) and Recombinant Rat IL‑1 alpha /IL‑1F1 (Catalog # ...read more
IL‑1 alpha /IL‑1F1 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 15 µg/mL ...read more
Recombinant Mouse IL‑1 alpha /IL‑1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IL-1 alpha/IL-1F1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL‑1 alpha /IL‑1F1
Ser6-Ser161
Accession # Q62161
Specificity
Detects mouse IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat IL‑1 alpha  and recombinant cotton rat IL‑1 alpha  is observed and less than 1% cross-reactivity with recombinant human IL‑1 alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1A
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.002-0.004 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF-400-NA in the following applications:

Publications
Read Publications using
AF-400-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody [Unconjugated]

  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 alpha/IL-1F1 Antibody (AF-400-NA)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IHC-Fr, Neutralization, Western Blot.


Filter By Application
IHC-Fr
(1)
Neutralization
(3)
Western Blot
(7)
All Applications
Filter By Species
Mouse
(11)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF-400-NA Applications Species
J Zhong, L Peng, B Wang, H Zhang, S Li, R Yang, Y Deng, H Huang, J Yuan Tacrolimus interacts with voriconazole to reduce the severity of fungal keratitis by suppressing IFN-related inflammatory responses and concomitant FK506 and voriconazole treatment suppresses fungal keratitis Mol. Vis., 2018;24(0):187-200. 2018 [PMID: 29527115] (Western Blot, Mouse) Western Blot Mouse
MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects J. Exp. Med., 2017;214(5):1297-1311. 2017 [PMID: 28400473] (Western Blot, Mouse) Western Blot Mouse
H Youssef, P Stashenko Interleukin-1 and estrogen protect against disseminating dentoalveolar infections Int J Oral Sci, 2017;9(1):16-23. 2017 [PMID: 28358036] (Neutralization, Mouse) Neutralization Mouse
Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3. Proc Natl Acad Sci U S A, 2015;112(13):4050-5. 2015 [PMID: 25775556] (IHC-Fr, Mouse) IHC-Fr Mouse
Caffrey A, Lehmann M, Zickovich J, Espinosa V, Shepardson K, Watschke C, Hilmer K, Thammahong A, Barker B, Rivera A, Cramer R, Obar J IL-1alpha signaling is critical for leukocyte recruitment after pulmonary Aspergillus fumigatus challenge. PLoS Pathog, 2015;11(1):e1004625. 2015 [PMID: 25629406] (Neutralization, Mouse) Neutralization Mouse
Ainscough J, Frank Gerberick G, Zahedi-Nejad M, Lopez-Castejon G, Brough D, Kimber I, Dearman R Dendritic cell IL-1alpha and IL-1beta are polyubiquitinated and degraded by the proteasome. J Biol Chem, 2014;289(51):35582-92. 2014 [PMID: 25371210] (Western Blot, Mouse) Western Blot Mouse
Uri-Belapolsky S, Shaish A, Eliyahu E, Grossman H, Levi M, Chuderland D, Ninio-Many L, Hasky N, Shashar D, Almog T, Kandel-Kfir M, Harats D, Shalgi R, Kamari Y Interleukin-1 deficiency prolongs ovarian lifespan in mice. Proc Natl Acad Sci U S A, 2014;111(34):12492-7. 2014 [PMID: 25114230] (Western Blot, Mouse) Western Blot Mouse
Asrat S, Dugan A, Isberg R The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PLoS Pathog, 2014;10(7):e1004229. 2014 [PMID: 25058342] (Western Blot, Mouse) Western Blot Mouse
Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. J. Immunol., 2011;187(9):4835-43. 2011 [PMID: 21930960] (Western Blot, Mouse) Western Blot Mouse
Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes. Nat. Immunol., 2010;11(10):897-904. 2010 [PMID: 20835230] (Western Blot, Mouse) Western Blot Mouse
Show All 11 Publications.

Review for IL-1 alpha/IL-1F1 Antibody (AF-400-NA) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF-400-NA:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen IL-1 alpha/IL-1F1 AF-400-NA
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reviewed by:
Chao Xiong
IHC-Fr Mouse 03/14/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested4% PFA-PBS fixed frozen sections from murine brain
SpeciesMouse
LotAMG0214111

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF400

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Recent Reviews

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5
1
4
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2
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1
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Chao Xiong
03/14/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol IL1A
Uniprot