IL-1 alpha/IL-1F1 Antibody [Unconjugated]

Western blot shows 50 ng of Recombinant Mouse IL-1a/IL-1F1 (400-ML), Recombinant Human IL-1a/IL-1F1 (200-LA) and Recombinant Rat IL-1a/IL-1F1 (500-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for IL-1a/IL-1F1 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

IL-1a/IL-1F1 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Recombinant Mouse IL-1a/IL-1F1 (400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA). The ND50 is typically 0.002‑0.004 µg/mL.

IL‑1 alpha /IL‑1F1 was detected in immersion fixed paraffin-embedded sections of Mouse Liver using Goat Anti-Mouse IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-400-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Experimental stroke-induced IL-1 expression is increased in the brain after LPS administration and anaphylaxis. Quantification of IL-1 alpha -positive cells (A) and granulocytes (B) in the ipsilateral hemisphere. C. Immunofluorescence shows IL-1 alpha -positive cells (green), granulocytes (red) and tomato lectin-positive blood vessels (blue) and microglia/macrophage cells in the ipsilateral cerebral cortex and thalamus after 24 h reperfusion. Perivascular cells in the cerebral cortex (i) and parenchymal cells in the thalamus (ii) expressing IL-1 alpha after MCAo are abundant in mice with anaphylaxis and LPS treatment, respectively. D. In situ hybridization reveals increased number of cells expressing IL-1 beta mRNA in the ischaemic cerebral cortex of mice 24 h after MCAo with anaphylaxis (Ova+A) compared to MCAo and Ova sensitization (Ova). E. Silver grains indicate the presence of IL-1 beta mRNA in Ova and Ova+A mice after MCAo (counterstained with cresyl violet). Inserts show higher magnification of IL-1 beta mRNA containing cells indicated by arrowheads. Data are representative of 4-5 mice in each group. One-way ANOVA followed by Bonferroni's comparison (P < 0.05 vs * Control, # Ova, $ Ova+A). Scale bars: Cii - 20 μm, C - 50 μm, E - 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22114895), licensed under a CC-BY license. Not internally tested by R&D Systems.

Experimental stroke-induced IL-1 expression is increased in the brain after LPS administration and anaphylaxis. Quantification of IL-1 alpha -positive cells (A) and granulocytes (B) in the ipsilateral hemisphere. C. Immunofluorescence shows IL-1 alpha -positive cells (green), granulocytes (red) and tomato lectin-positive blood vessels (blue) and microglia/macrophage cells in the ipsilateral cerebral cortex and thalamus after 24 h reperfusion. Perivascular cells in the cerebral cortex (i) and parenchymal cells in the thalamus (ii) expressing IL-1 alpha after MCAo are abundant in mice with anaphylaxis and LPS treatment, respectively. D. In situ hybridization reveals increased number of cells expressing IL-1 beta mRNA in the ischaemic cerebral cortex of mice 24 h after MCAo with anaphylaxis (Ova+A) compared to MCAo and Ova sensitization (Ova). E. Silver grains indicate the presence of IL-1 beta mRNA in Ova and Ova+A mice after MCAo (counterstained with cresyl violet). Inserts show higher magnification of IL-1 beta mRNA containing cells indicated by arrowheads. Data are representative of 4-5 mice in each group. One-way ANOVA followed by Bonferroni's comparison (P < 0.05 vs * Control, # Ova, $ Ova+A). Scale bars: Cii - 20 μm, C - 50 μm, E - 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22114895), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-1 alpha/IL-1F1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑1 alpha /IL‑1F1
  Ser6-Ser161
  Accession # Q62161
• Specificity: Detects mouse IL‑1 alpha /IL‑1F1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat IL‑1 alpha and recombinant cotton rat IL‑1 alpha is observed and less than 1% cross-reactivity with recombinant human IL‑1 alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1A
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Neutralization 0.002-0.004 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody [Unconjugated]

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

IL-1 is a name that designates two proteins, IL-1 alpha  and IL-1 beta , that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta , designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.

IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Two distinct types of IL-1 receptors have been identified and cloned from human and mouse cells. The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. The IL-1 receptor type II is a 68 kDa membrane protein with a relatively short cytoplasmic tail and has no signaling function. The type II receptor acts as a decoy target for IL-1, inhibiting IL-1 activities by preventing the binding of IL-1 to the type I receptor. A soluble version of the type II receptor is induced by anti‑inflammatory agents such as glucocorticoids, IL-4 and IL-13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 alpha/IL-1F1 Antibody (AF-400-NA)(33)

Publications using AF-400-NA

• Zhong, X;Wu, H;Zhang, W;Shi, Y;Gwack, Y;Xue, HH;Sun, Z; Distinct ROR?t-dependent Th17 immune responses are required for autoimmune pathogenesis and protection against bacterial infection Cell reports 2024-11-05 [PMID: 39504243] (Neutralization, Mouse)
• Lorenzo-Martín, LF;Hübscher, T;Bowler, AD;Broguiere, N;Langer, J;Tillard, L;Nikolaev, M;Radtke, F;Lutolf, MP; Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo Nature 2024-04-24 [PMID: 38658753] (Immunohistochemistry, Transgenic Mouse)
• Membrane-bound Interleukin-1 alpha mediates leukocyte adhesion during atherogenesis [PMID: 37701434] (Western Blot, Mouse)
• FR Millar, A Pennycuick, M Muir, A Quintanill, P Hari, E Freyer, P Gautier, A Meynert, G Grimes, CS Coll, S Zdral, S Victorelli, VH Teixeira, J Connelly, JF Passos, MA Ros, WAH Wallace, MC Frame, AH Sims, L Boulter, SM Janes, S Wilkinson, JC Acosta Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer Cell Reports, 2022-11-08;41(6):111596. 2022-11-08 [PMID: 36351380] (IHC, Transgenic Mouse)
• Bretheau F, Castellanos-Molina A, B�langer D et al. The alarmin interleukin-1? triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury Nature Communications 2022-10-02 [PMID: 36184639]
• Christopher Hoyle, Jack P. Green, Stuart M. Allan, David Brough, Eloise Lemarchand Itaconate and fumarate derivatives inhibit priming and activation of the canonical NLRP3 inflammasome in macrophages Immunology 2022-04-01 [PMID: 35137954]
• A Matsunami, M Mizuno-Kam, H Kawaki, E Takayama, K Ueno, M Ando, H Morimoto-I, Y Muramatsu, S Sumitomo, N Kondoh Augmented secretion of IL-1&amp;alpha from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells Journal of oral biosciences, 2021-06-18;0(0):. 2021-06-18 [PMID: 34153475] (Functional Assay, Mouse)
• K Tsuchiya, S Hosojima, H Hara, H Kushiyama, MR Mahib, T Kinoshita, T Suda Gasdermin D mediates the maturation and release of IL-1&amp;alpha downstream of inflammasomes Cell Reports, 2021-03-23;34(12):108887. 2021-03-23 [PMID: 33761363] (Western Blot, Mouse)
• Melchor, S J, Hatter, J A Et al. T. gondii infection induces IL-1R dependent chronic cachexia and perivascular fibrosis in the liver and skeletal muscle. Sci Rep 9/24/2020 [PMID: 32973293]
• Surendra K. Shukla, Spas D. Markov, Kuldeep S. Attri, Enza Vernucci, Ryan J. King, Aneesha Dasgupta et al. Macrophages potentiate STAT3 signaling in skeletal muscles and regulate pancreatic cancer cachexia Cancer Letters 2020-08-01 [PMID: 32344015]
• M Speir, CJ Nowell, AA Chen, JA O'Donnell, IS Shamie, PR Lakin, AA D'Cruz, RO Braun, JJ Babon, RS Lewis, M Bliss-More, I Shlomovitz, S Wang, LH Cengia, AI Stoica, R Hakem, MA Kelliher, LA O'Reilly, H Patsiouras, KE Lawlor, E Weller, NE Lewis, AW Roberts, M Gerlic, BA Croker Ptpn6 inhibits caspase-8- and Ripk3/Mlkl-dependent inflammation Nat. Immunol., 2019-12-09;21(1):54-64. 2019-12-09 [PMID: 31819256] (Western Blot, Mouse)
• Hong‐Ming Zhou, Radomir M. Slominski, Leroy J. Seymour, Maria C. Bell, Priya Dave, Joseph Atumonye et al. Ex vivo culture of mouse skin activates an interleukin 1 alpha‐dependent inflammatory response Experimental Dermatology 2020-01-01 [PMID: 31566815] (Immunohistochemistry, Mouse)
• Brittany Butler, Robyn De Dios, Leanna Nguyen, Sarah McKenna, Sankar Ghosh, Clyde J. Wright Developmentally Regulated Innate Immune NF kappa B Signaling Mediates IL-1 alpha Expression in the Perinatal Murine Lung Frontiers in Immunology 2019-07-10 [PMID: 31354715]
• VS Tapia, MJD Daniels, P Palazón-Ri, M Dewhurst, NM Luheshi, J Rivers-Aut, J Green, E Redondo-Ca, P Kaldis, G Lopez-Cast, D Brough The three cytokines IL-1?, IL-18, and IL-1? share related but distinct secretory routes J. Biol. Chem., 2019-04-02;294(21):8325-8335. 2019-04-02 [PMID: 30940725] (Western Blot, Mouse)
• J Zhong, L Peng, B Wang, H Zhang, S Li, R Yang, Y Deng, H Huang, J Yuan Tacrolimus interacts with voriconazole to reduce the severity of fungal keratitis by suppressing IFN-related inflammatory responses and concomitant FK506 and voriconazole treatment suppresses fungal keratitis Mol. Vis., 2018-03-04;24(0):187-200. 2018-03-04 [PMID: 29527115] (Western Blot, Mouse)
• Megan Osbourn, Dinesh C. Soares, Francesco Vacca, E. Suzanne Cohen, Ian C. Scott, William F. Gregory et al. HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33 Immunity 2017-10-17 [PMID: 29045903]
• MK Choo, Y Sano, C Kim, K Yasuda, XD Li, X Lin, M Stenzel-Po, L Alexopoulo, S Ghosh, E Latz, IR Rifkin, ZJ Chen, GC Stewart, H Chong, JM Park TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects J. Exp. Med., 2017-04-11;214(5):1297-1311. 2017-04-11 [PMID: 28400473] (Western Blot, Mouse)
• H Youssef, P Stashenko Interleukin-1 and estrogen protect against disseminating dentoalveolar infections Int J Oral Sci, 2017-03-01;9(1):16-23. 2017-03-01 [PMID: 28358036] (Neutralization, Mouse)
• Weiling Yu, Stephan Bien-Aime, Marcelo Mattos, Sarah Alsadun, Keisuke Wada, Sarah Rogado et al. Sustained, localized salicylic acid delivery enhances diabetic bone regeneration via prolonged mitigation of inflammation Journal of Biomedical Materials Research Part A 2016-10-01 [PMID: 27194511]
• Sadeghi H, Lockmann A, Hund A, Samavedam U, Pipi E, Vafia K, Hauenschild E, Kalies K, Pas H, Jonkman M, Iwata H, Recke A, Schon M, Zillikens D, Schmidt E, Ludwig R Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules. J Immunol, 2015-03-20;194(8):3656-63. 2015-03-20 [PMID: 25795756] (Bioassay, Mouse)
• Denes A, Coutts G, Lenart N, Cruickshank S, Pelegrin P, Skinner J, Rothwell N, Allan S, Brough D AIM2 and NLRC4 inflammasomes contribute with ASC to acute brain injury independently of NLRP3. Proc Natl Acad Sci U S A, 2015-03-16;112(13):4050-5. 2015-03-16 [PMID: 25775556] (IHC-Fr, Mouse)
• Caffrey A, Lehmann M, Zickovich J, Espinosa V, Shepardson K, Watschke C, Hilmer K, Thammahong A, Barker B, Rivera A, Cramer R, Obar J IL-1alpha signaling is critical for leukocyte recruitment after pulmonary Aspergillus fumigatus challenge. PLoS Pathog, 2015-01-28;11(1):e1004625. 2015-01-28 [PMID: 25629406] (Neutralization, Mouse)
• Ainscough J, Frank Gerberick G, Zahedi-Nejad M, Lopez-Castejon G, Brough D, Kimber I, Dearman R Dendritic cell IL-1alpha and IL-1beta are polyubiquitinated and degraded by the proteasome. J Biol Chem, 2014-11-04;289(51):35582-92. 2014-11-04 [PMID: 25371210] (Western Blot, Mouse)
• Uri-Belapolsky S, Shaish A, Eliyahu E, Grossman H, Levi M, Chuderland D, Ninio-Many L, Hasky N, Shashar D, Almog T, Kandel-Kfir M, Harats D, Shalgi R, Kamari Y Interleukin-1 deficiency prolongs ovarian lifespan in mice. Proc Natl Acad Sci U S A, 2014-08-11;111(34):12492-7. 2014-08-11 [PMID: 25114230] (Western Blot, Mouse)
• Asrat S, Dugan A, Isberg R The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines. PLoS Pathog, 2014-07-24;10(7):e1004229. 2014-07-24 [PMID: 25058342] (Western Blot, Mouse)
• Krisztián Pajer, Georg A. Feichtinger, Gábor Márton, Sonja Sabitzer, Dieter Klein, Heinz Redl et al. Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die Experimental Neurology 2014-11-01 [PMID: 24907401]
• Bryce N. Alves, Rachel Tsui, Jonathan Almaden, Maxim N. Shokhirev, Jeremy Davis-Turak, Jessica Fujimoto et al. I kappa B epsilon Is a Key Regulator of B Cell Expansion by Providing Negative Feedback on cRel and RelA in a Stimulus-Specific Manner The Journal of Immunology 2014-04-01 [PMID: 24591377]
• John S. Lewis, Bridgette D. Furman, Evan Zeitler, Janet L. Huebner, Virginia B. Kraus, Farshid Guilak et al. Genetic and cellular evidence of decreased inflammation associated with reduced incidence of posttraumatic arthritis in MRL/MpJ mice Arthritis & Rheumatism 2013-06-27 [PMID: 23203659]
• Nadia M Luheshi, Krisztina J Kovács, Gloria Lopez-Castejon, David Brough, Adam Denes Interleukin-1 alpha expression precedes IL-1 beta after ischemic brain injury and is localised to areas of focal neuronal loss and penumbral tissues Journal of Neuroinflammation 2011-12-29 [PMID: 22206506]
• Ádám Dénes, Szilamér Ferenczi, Krisztina J Kovács Systemic inflammatory challenges compromise survival after experimental stroke via augmenting brain inflammation, blood- brain barrier damage and brain oedema independently of infarct size Journal of Neuroinflammation 2011-11-24 [PMID: 22114895]
• Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. J. Immunol., 2011-09-19;187(9):4835-43. 2011-09-19 [PMID: 21930960] (ICC, IHC-Fr, Neutralization, Western Blot, Mouse)
• Masters SL, Dunne A, Subramanian SL, Hull RL, Tannahill GM, Sharp FA, Becker C, Franchi L, Yoshihara E, Chen Z, Mullooly N, Mielke LA, Harris J, Coll RC, Mills KH, Mok KH, Newsholme P, Nunez G, Yodoi J, Kahn SE, Lavelle EC, O'Neill LA Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a mechanism for enhanced IL-1beta in type 2 diabetes. Nat. Immunol., 2010-09-12;11(10):897-904. 2010-09-12 [PMID: 20835230] (Western Blot, Mouse)
• Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening. Bone, 2006-12-21;40(5):1276-83. 2006-12-21 [PMID: 17236833] (Neutralization, Mouse)

Review for IL-1 alpha/IL-1F1 Antibody (AF-400-NA) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Chao Xiong IHC-Fr Mouse 03/14/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: 4% PFA-PBS fixed frozen sections from murine brain
• Species: Mouse
• Lot: AMG0214111

Bioinformatics

• Gene Symbol: IL1A
• Uniprot:
  • Q62161()