IL-1 alpha/IL-1F1 Antibody [Unconjugated]

Images

 
Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more
IL‑1 alpha /IL‑1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS using Goat Anti-Human IL‑1 alpha /IL‑1F1 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

IL-1 alpha/IL-1F1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-1 alpha /IL-1F1
Ser113-Ala271
Accession # Q53QF9
Specificity
Detects human IL-1 alpha /IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) IL-1 alpha and recombinant rat IL‑1 alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1A
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 4-20 ng/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF-200-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody [Unconjugated]

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta , which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha . The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF-200-NA
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Applications: WB, B/N, ICC/IF

Publications for IL-1 alpha/IL-1F1 Antibody (AF-200-NA)(8)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 6 applications: Bioassay, ICC, IHC-P, Inhibition, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ICC
(2)
IHC-P
(1)
Inhibition
(1)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF-200-NA Applications Species
D Liu, A Steins, R Klaassen, AP van der Za, RJ Bennink, G van Tienho, MG Besselink, MF Bijlsma, HWM van Laarho Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma Biomedicines, 2020;8(11):. 2020 [PMID: 33105540] (Inhibition, Human) Inhibition Human
L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis Autophagy, 2017;0(0):0. 2017 [PMID: 28933598] (Neutralization, Human) Neutralization Human
Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients PLoS ONE, 2016;11(9):e0162908. 2016 [PMID: 27649540] (Bioassay, Human) Bioassay Human
Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Br. J. Pharmacol., 2007;151(1):115-27. 2007 [PMID: 17351655] (Western Blot, Human) Western Blot Human
Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK The response of human epithelial cells to TNF involves an inducible autocrine cascade. Cell, 2006;124(6):1225-39. 2006 [PMID: 16564013] (Neutralization, Human) Neutralization Human
Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1). Cytokine, 2005;31(2):153-60. 2005 [PMID: 15923123] (ICC, Human) ICC Human
Sengupta J, Dhawan L, Ghosh D Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey. Cytokine, 2003;24(6):277-85. 2003 [PMID: 14609569] (IHC-P, Primate - Macaca mulatta (Rhesus Macaque)) IHC-P Primate - Macaca mulatta (Rhesus Macaque)
Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha. Circ. Res., 2003;93(6):515-22. 2003 [PMID: 12946945] (ICC, Human) ICC Human

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF200

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Bioinformatics

Gene Symbol IL1A
Uniprot