IL-1 alpha/IL-1F1 Antibody [Unconjugated]


Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human more
IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS using Goat Anti-Human IL-1a/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) at 10 more
TPM1 and TPM4 expression in response to IL-1 alpha .High molecular weight tropomyosin isoform expression in THP1 cells in response to elevated concentrations of IL-1 alpha . Image collected and cropped by CiteAb from more

Product Details

Reactivity HuSpecies Glossary
Applications WB, B/N, ICC/IF

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

IL-1 alpha/IL-1F1 Antibody [Unconjugated] Summary

E. coli-derived recombinant human IL-1 alpha /IL-1F1
Accession # Q53QF9
Detects human IL-1 alpha /IL-1F1 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 4-20 ng/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF-200-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 alpha/IL-1F1 Antibody [Unconjugated]

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha


Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta , which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha . The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 alpha/IL-1F1 Antibody (AF-200-NA)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 7 applications: Bioassay, ELISA Capture, ICC, IHC-P, Inhibition, Neutralization, Western Blot.

Filter By Application
ELISA Capture
Western Blot
All Applications
Filter By Species
Primate - Macaca mulatta (Rhesus Macaque)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF-200-NA Applications Species
Smer-Barreto, V;Quintanilla, A;Elliott, RJR;Dawson, JC;Sun, J;Campa, VM;Lorente-Macías, Á;Unciti-Broceta, A;Carragher, NO;Acosta, JC;Oyarzún, DA; Discovery of senolytics using machine learning Nature communications 2023-06-10 [PMID: 37301862] (ICC, Human) ICC Human
Ming-Na Zhao, Ling-Fei Zhang, Zhen Sun, Li-Hua Qiao, Tao Yang, Yi-Zhe Ren et al. A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer Cell Death & Disease 5/1/2023 [PMID: 37127752]
YY Chao, A Puhach, D Frieser, M Arunkumar, L Lehner, T Seeholzer, A Garcia-Lop, M van der Wa, S Fibi-Smeta, A Dietschman, T Sommermann, T ?ikovi?, L Taher, MS Gresnigt, SJ Vastert, F van Wijk, G Panagiotou, D Krappmann, O Gro beta , CE Zielinski Human TH17 cells engage gasdermin E pores to release IL-1alpha on NLRP3 inflammasome activation Nature Immunology, 2023-01-05;0(0):. 2023-01-05 [PMID: 36604548] (ICC, Human) ICC Human
Haoran Zhu, Keefe T Chan, Xinran Huang, Carmelo Cerra, Shaun Blake, Anna S Trigos et al. Cystathionine-beta -synthase is essential for AKT-induced senescence and suppresses the development of gastric cancers with PI3K/AKT activation eLife 2022-06-27 [PMID: 35758651]
Z Pan, Y Gao, S Liu, Z Ke, J Guo, W Ma, T Cui, B Liu, X Zhang Wu-Zi-Yan-Zong-Wan protects mouse blood-testis barrier from Tripterygium wilfordii Hook. f. multiglycoside-induced disruption by regulating proinflammatory cytokines Journal of ethnopharmacology, 2021-07-19;280(0):114440. 2021-07-19 [PMID: 34293456] (ELISA Capture, Mouse) ELISA Capture Mouse
D Liu, A Steins, R Klaassen, AP van der Za, RJ Bennink, G van Tienho, MG Besselink, MF Bijlsma, HWM van Laarho Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma Biomedicines, 2020-10-22;8(11):. 2020-10-22 [PMID: 33105540] (Inhibition, Human) Inhibition Human
J Sun, DL LaRock, EA Skowronski, JM Kimmey, J Olson, Z Jiang, AJ O'Donoghue, V Nizet, CN LaRock The Pseudomonas aeruginosa protease LasB directly activates IL-1&amp;beta EBioMedicine, 2020-09-23;60(0):102984. 9/23/2020 [PMID: 32979835]
L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis Autophagy, 2017-01-01;0(0):0. 2017-01-01 [PMID: 28933598] (Neutralization, Human) Neutralization Human
Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients PLoS ONE, 2016-09-20;11(9):e0162908. 2016-09-20 [PMID: 27649540] (Bioassay, Human) Bioassay Human
A T R-Viso, M I S Duarte, C Pagliari, E R Fernandes, R A Brasil, G Benard et al. Tissue and serum immune response in chronic hepatitis C with mild histological lesions Memórias do Instituto Oswaldo Cruz 2010-02-01 [PMID: 20209325]
Show All 15 Publications.

Reviews for IL-1 alpha/IL-1F1 Antibody (AF-200-NA) (0)

There are no reviews for IL-1 alpha/IL-1F1 Antibody (AF-200-NA). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-1 alpha/IL-1F1 Antibody (AF-200-NA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our IL-1 alpha/IL-1F1 Antibody [Unconjugated] and receive a gift card or discount.


Gene Symbol IL1A