IL-1 alpha/IL-1F1 Antibody Pair [HRP]

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Sandwich ELISA: IL-1 alpha/IL-1F1 Antibody Pair [HRP] [NBP2-79522] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, S-ELISA
Conjugate
HRP
Standard Curve Range
6.25-400 pg/ml
Sensitivity
6.25 pg/ml

Order Details

IL-1 alpha/IL-1F1 Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Mouse IL-1 alpha/IL-1F1.
Specificity
This solid phase sandwich ELISA utilizes an antibody specific for Mouse IL-1 alpha/IL-1F1.
Standard Curve Range
6.25-400 pg/ml
Sensitivity
6.25 pg/ml
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Gene
IL1A

Applications/Dilutions

Application Notes
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. Detection Antibody: 0.2 mg/ml of rabbit anti-IL-1 alpha/IL-1F1 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  2. Capture Antibody: 0.5 mg/ml of mouse anti-Mouse IL-1 alpha/IL-1F1 monoclonal antibody (in PBS, pH 7.4).
  3. Standard: Each vial contains 30 ng of recombinant Mouse IL-1 alpha/IL-1F1.

Alternate Names for IL-1 alpha/IL-1F1 Antibody Pair [HRP]

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Other Available Formats

HRP NBP2-79522

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Bioinformatics

Gene Symbol IL1A