IKK gamma Antibody [Unconjugated]

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human IKK gamma (1 ng) was included. Specific bands for IKK gamma were detected at approximately 50 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of MDA‑MB‑468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for IKK gamma at approximately 47-56 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

IKK gamma was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IKK gamma Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IKK gamma
  Met1-Glu419
  Accession # Q9Y6K9
• Specificity: Detects human, mouse, and rat IKK gamma in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IKBKG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IKK gamma Antibody [Unconjugated]

• FIP-3IP2
• FIP3NF-kappa-B essential modifier
• FIP3P
• I-kappa-B kinase subunit gamma
• IkB kinase gamma subunit
• IkB kinase subunit gamma
• IkB kinase-associated protein 1
• IKBKG
• IKK gamma
• IKKAP1
• IKKG
• IKK-gammaIPD2
• incontinentia pigmenti
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Inhibitor of nuclear factor kappa-B kinase subunit gamma
• IP
• IP1
• NEMO
• NEMOAMCBX1
• NFkappaB essential modulator
• NF-kappa-B essential modulator

Background

I kappa B kinase gamma (IKK gamma ) is also known as NF-kappa B essential modulator (NEMO), IKBKG, and FIP3. The active IKK complex, composed of IKK alpha , IKK beta , and two forms of processed IKK gamma (50 and 55 kDa), phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. The regulatory IKK gamma is required for activation of the IKK complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IKK gamma Antibody (AF2684)(1)

Publication using AF2684

• L Wang, H Ma, Y Xue, H Shi, T Ma, X Cui Berberine inhibits the ischemia-reperfusion injury induced inflammatory response and apoptosis of myocardial cells through the phosphoinositide 3-kinase/RAC-? serine/threonine-protein kinase and nuclear factor-?B signaling pathways Exp Ther Med, 2017-11-27;15(2):1225-1232. 11/27/2017 [PMID: 29403554]

Bioinformatics

• Gene Symbol: IKBKG
• Uniprot:
  • Q9Y6K9()