IKB zeta Antibody - BSA Free

Western Blot: IKB zeta Antibody - BSA Free [NBP3-47347] - HeLa cells were subjected to SDS PAGE followed by western blot with (IKB zeta antibody) at dilution of 1:600

Immunohistochemistry: IKB zeta Antibody - BSA Free [NBP3-47347] - Immunohistochemistry of paraffin-embedded mouse heart using (IKB zeta antibody) at dilution of 1:50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IKB zeta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IKB zeta Antibody - BSA Free (NBP3-47347) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta. (Uniprot# Q9BYH8)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFKBIZ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for IKB zeta Antibody - BSA Free

• FLJ30225
• FLJ34463
• ikappaBzeta
• I-kappa-B-zeta
• IkappaB-zeta
• ikbzeta
• IkB-zeta
• IL-1 inducible nuclear ankyrin-repeat protein
• INAPIkappa B-zeta variant 3
• MAILIKBZikB-zeta
• Molecule possessing ankyrin repeats induced by lipopolysaccharide
• NF-kappa-B inhibitor zeta
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

IkappaBzeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFKBIZ