IkB-epsilon Antibody

Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Mouse IkB-e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4637) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IkB-e at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

IkB-epsilon Antibody Summary

• Immunogen: E. coli-derived recombinant mouse I kappa B-epsilon
  Met1-Asp364
  Accession # O54910
• Specificity: Detects endogenous mouse IkB-epsilon in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NFKBIE
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IkB-epsilon Antibody

• epsilon
• ikappaBepsilon
• I-kappa-B-epsilon
• IkB-E
• IKBENF-kappa-B inhibitor epsilon
• IkBepsilon
• IkB-epsilon
• NF-kappa-BIE
• NFKBIE
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF kappa B inhibitor epsilon (IkB-epsilon ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-epsilon is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-epsilon degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IkB-epsilon Antibody (AF4637)(1)

Publication using AF4637

• Sarah Evans, Huei-Ping Tzeng, Deborah J. Veis, Scot Matkovich, Carla Weinheimer, Attila Kovacs et al. TNF receptor–activated factor 2 mediates cardiac protection through noncanonical NF-kappa B signaling JCI Insight 2018-02-08 [PMID: 29415884]

Bioinformatics

• Gene Symbol: NFKBIE
• Uniprot:
  • O54910()