Ikaros/IKZF1 Antibody

Images

 
Western Blot: Ikaros/IKZF1 Antibody [NBP1-51990] - MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] - NBP1-51990 (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Ikaros/IKZF1 Antibody Summary

Immunogen
Peptide with sequence C-DTPYDSSASYEKEN corresponding to internal region according to NP_006051.1.
Epitope
C-DTPYDSSASYEKEN
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IKZF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Approx. 65 kDa band observed in MOLT4 lysates (calculated MW of 57.5 kDa band according to NP_006051.1). Similar results were obtained with NBP1-00125, which was developed against a different part of the protein. IHC-P: Human tonsil shows textured staining in some nuclei of the lymphoid tissue.
Publications
Read Publication using NBP1-51990.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ikaros/IKZF1 Antibody

  • DNA-binding protein Ikaros
  • hIk-1
  • IK1
  • IK1LyF-1
  • Ikaros (zinc finger protein)
  • IKAROS family zinc finger 1 (Ikaros)
  • Ikaros family zinc finger protein 1
  • Ikaros
  • IKAROSLymphoid transcription factor LyF-1
  • IKZF1
  • LYF1
  • LyF-1
  • LYF1PRO0758
  • PRO0758
  • zinc finger protein, subfamily 1A, 1 (Ikaros)
  • ZNFN1A1
  • ZNFN1A1CLL-associated antigen KW-6

Background

IKZF1 is a transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ikaros/IKZF1 Antibody (NBP1-51990)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IKZF1
Entrez
Uniprot