Rat IGFBP-3 ELISA Kit (Colorimetric)

ELISA: Rat IGFBP-3 ELISA Kit (Colorimetric) [NBP3-18774] - Samples were spiked with high concentrations of Rat IGFBP-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat IGFBP-3 ELISA Kit (Colorimetric) [NBP3-18774] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL

Rat IGFBP-3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat IGFBP-3 in samples. No significant cross-reactivity or interference between Rat IGFBP-3 and analogues was observed.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.79
• Intra-Assay: %CV < 5.8
• Spike Recovery: 86-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IGFBP3

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat IGFBP-3 ELISA Kit (Colorimetric)

• acid stable subunit of the 140 K IGF complex
• binding protein 29
• binding protein 53
• growth hormone-dependent binding protein
• IBP-3
• IBP3BP-53
• IGF-binding protein 3
• IGFBP3
• IGFBP-3
• insulin-like growth factor binding protein 3
• insulin-like growth factor-binding protein 3

Background

IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IGFBP3