Human IGFBP-1 Recombinant Protein

Product Discontinued

Human IGFBP-1 Recombinant Protein Summary

• Description: A single glycosylated polypeptide protein corresponding to amino acids Ala26 - Asn259 IGFBP1 (P08833).
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Lyophilized from a 0.2 um filtered concentrated solution in acetonitrile and TFA.
• Details of Functionality: The ED50, as determined by the inhibition of rhIGF-I-induced proliferation of MCF-7 cells, is less than 4 mg/mL.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: IGFBP1
• Endotoxin Note: Less than 1 EU/ug of rHuIGFBP-1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with sterilized PBS to a final concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human IGFBP-1 Recombinant Protein

• alpha-pregnancy-associated endometrial globulin
• amniotic fluid binding protein
• binding protein-25
• binding protein-26
• binding protein-28
• growth hormone independent-binding protein
• hIGFBP-1
• IBP1
• IBP-1
• IGF-binding protein 1
• IGFBP1
• IGFBP-1
• IGF-BP25
• insulin-like growth factor binding protein 1
• insulin-like growth factor-binding protein 1
• Placental protein 12
• PP12AFBP

Background

IGFBP-1 is a secreted protein that binds and regulates the bioavailability of IGF-I and IGF-II. It is primarily expressed in the liver and kidney in response to changes in plasma insulin concentrations. IGFBP-1 inhibits IGF action in bone and the IGF-I induced hypoglycemic response. Dysregulated IGFBP-1 levels are linked to insulin resistance and the development of diabetes, coronary heart disease and acute myocardial infarction, and the progression of prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IGFBP1