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Human IGFBP-1 ELISA Kit (Colorimetric)

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ELISA: Human IGFBP-1 ELISA Kit (Colorimetric) [KA1872] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human IGFBP-1 ELISA Kit (Colorimetric) [KA1872] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum, Urine
Sensitivity
5 pg/mL

Order Details

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Human IGFBP-1 ELISA Kit (Colorimetric) Summary

Sensitivity
5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IGFBP1

Applications/Dilutions

Application Notes
IGFBP1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP1.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): Diluent buffer contains 0.09% sodium azide as preservative.
  2. Assay Diluent B (Item E): 5x concentrated buffer.
  3. HRP-Streptavidin Concentrate (Item G): 240x concentrated HRP-conjugatedstreptavidin.
  4. IGFBP-1 Microplate (Item A): 96 wells coated with anti-Human IGFBP-1.
  5. Detection Antibody IGFBP-1 (Item F): Biotinylated anti-Human IGFBP-1 (each vial isenough to assay half microplate).
  6. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
  7. TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine (TMB) inbuffered solution.
  8. Standard Protein (Item C): Human IGFBP-1, I vial is enough to run each standard induplicate.
  9. Stop Solution (Item I): 0.2 M sulfuric acid.
  10. Note: Return unused wells to the pouch containing desiccant pack, reseal along entire edge.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human IGFBP-1 ELISA Kit (Colorimetric)

  • alpha-pregnancy-associated endometrial globulin
  • amniotic fluid binding protein
  • binding protein-25
  • binding protein-26
  • binding protein-28
  • growth hormone independent-binding protein
  • hIGFBP-1
  • IBP1
  • IBP-1
  • IGF-binding protein 1
  • IGFBP1
  • IGFBP-1
  • IGF-BP25
  • insulin-like growth factor binding protein 1
  • insulin-like growth factor-binding protein 1
  • Placental protein 12
  • PP12AFBP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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1 μg/lane of Recombinant Human IGF-I was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at&amp;nbsp;7 kDa.
Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5&amp;nbsp;ng/mL.
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Recombinant Human IGF-II (Catalog&amp;nbsp;# 292&amp;#8209;G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 1.5‑6&amp;nbsp;ng/mL.
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Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL of Goat Anti-Human IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). A specific band was detected for IGFBP‑2 at approximately 35&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for IGFBP‑2 at approximately 42&amp;nbsp;kDa (as indicated) using 25&amp;nbsp;µg/mL of Goat Anti-Human IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF674) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Recombinant Human IGFBP‑2 (Catalog&amp;nbsp;# 674-B2) inhibits Recombinant Human IGF‑II (Catalog&amp;nbsp;# 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑II (14&amp;nbsp;ng/mL) activity elicited by Recombinant Human IGFBP‑2 (0.2&amp;nbsp;µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGFBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF674). The ND50 is typically 2.5-7.5&amp;nbsp;µg/mL.
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Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). A specific band was detected for Growth Hormone at approximately 22&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Human IGFBP-1
ELISA Kit (Colorimetr
KA1872
Human IGFBP-1 ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

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Bioinformatics

Gene Symbol IGFBP1