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Human IGFBP-1 ELISA Kit (Colorimetric)

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ELISA: Human IGFBP-1 ELISA Kit (Colorimetric) [KA1872] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human IGFBP-1 ELISA Kit (Colorimetric) [KA1872] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum, Urine
Sensitivity
5 pg/mL

Order Details

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Human IGFBP-1 ELISA Kit (Colorimetric) Summary

Sensitivity
5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IGFBP1

Applications/Dilutions

Application Notes
IGFBP1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP1.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): Diluent buffer contains 0.09% sodium azide as preservative.
  2. Assay Diluent B (Item E): 5x concentrated buffer.
  3. HRP-Streptavidin Concentrate (Item G): 240x concentrated HRP-conjugatedstreptavidin.
  4. IGFBP-1 Microplate (Item A): 96 wells coated with anti-Human IGFBP-1.
  5. Note: Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
  6. Detection Antibody IGFBP-1 (Item F): Biotinylated anti-Human IGFBP-1 (each vial isenough to assay half microplate).
  7. Standard Protein (Item C): Human IGFBP-1, I vial is enough to run each standard induplicate.
  8. Stop Solution (Item I): 0.2 M sulfuric acid.
  9. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
  10. TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine (TMB) inbuffered solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human IGFBP-1 ELISA Kit (Colorimetric)

  • alpha-pregnancy-associated endometrial globulin
  • amniotic fluid binding protein
  • binding protein-25
  • binding protein-26
  • binding protein-28
  • growth hormone independent-binding protein
  • hIGFBP-1
  • IBP1
  • IBP-1
  • IGF-binding protein 1
  • IGFBP1
  • IGFBP-1
  • IGF-BP25
  • insulin-like growth factor binding protein 1
  • insulin-like growth factor-binding protein 1
  • Placental protein 12
  • PP12AFBP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5&amp;nbsp;ng/mL.
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Bioinformatics

Gene Symbol IGFBP1
Entrez