Human IGFBP-1 ELISA Kit (Colorimetric) Summary
| Sensitivity |
5 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 15% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IGFBP1 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
IGFBP1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP1. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): Diluent buffer contains 0.09% sodium azide as preservative.
- Assay Diluent B (Item E): 5x concentrated buffer.
- Detection Antibody IGFBP-1 (Item F): Biotinylated anti-Human IGFBP-1 (each vial isenough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 240x concentrated HRP-conjugatedstreptavidin.
- IGFBP-1 Microplate (Item A): 96 wells coated with anti-Human IGFBP-1.
- Note: Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
- Standard Protein (Item C): Human IGFBP-1, I vial is enough to run each standard induplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine (TMB) inbuffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human IGFBP-1 ELISA Kit (Colorimetric)
Background
IGFBP1 is a gene that codes for an IGF-binding protein that circulates in the plasma and prolongs the half-life of IGFs and works to either inhibit of stimulate the growth promoting effects while also promoting cell migration and has a length of 259 amino acids and a weight of approximately 28 kDa. Current studies are being done on several diseases and disorders relating to this gene including protein-energy malnutrition, pre-eclampsia, persistent fetal circulation syndrome, Silver-Russell syndrome, growth hormone deficiency, Ehlers-Danlos syndrome, glucose intolerance, Prader-Willi syndrome, and chronic fatigue syndrome. IGFBP1 has also been shown to have interactions with IGF1, IGF2, MMP26, TF, and ARNT in pathways such as the myometrial relaxation and contraction, HIF-1-alpha transcription factor, IGF regulation, and protein metabolism pathways.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IGFBP-1 ELISA Kit (KA1872). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.