Human IGFBP-1 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1872
    • Availability
      Product Discontinued

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Human IGFBP-1 ELISA Kit (Colorimetric) Summary

Sensitivity
5 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IGFBP1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
IGFBP1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP1.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A (Item D): Diluent buffer contains 0.09% sodium azide as preservative.
  2. Assay Diluent B (Item E): 5x concentrated buffer.
  3. Detection Antibody IGFBP-1 (Item F): Biotinylated anti-Human IGFBP-1 (each vial isenough to assay half microplate).
  4. HRP-Streptavidin Concentrate (Item G): 240x concentrated HRP-conjugatedstreptavidin.
  5. IGFBP-1 Microplate (Item A): 96 wells coated with anti-Human IGFBP-1.
  6. Note: Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
  7. Standard Protein (Item C): Human IGFBP-1, I vial is enough to run each standard induplicate.
  8. Stop Solution (Item I): 0.2 M sulfuric acid.
  9. TMB One-Step Substrate Reagent (Item H): 3,3’,5,5’-tetramethylbenzidine (TMB) inbuffered solution.
  10. Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human IGFBP-1 ELISA Kit (Colorimetric)

  • alpha-pregnancy-associated endometrial globulin
  • amniotic fluid binding protein
  • binding protein-25
  • binding protein-26
  • binding protein-28
  • growth hormone independent-binding protein
  • hIGFBP-1
  • IBP1
  • IBP-1
  • IGF-binding protein 1
  • IGFBP1
  • IGFBP-1
  • IGF-BP25
  • insulin-like growth factor binding protein 1
  • insulin-like growth factor-binding protein 1
  • Placental protein 12
  • PP12AFBP

Background

IGFBP1 is a gene that codes for an IGF-binding protein that circulates in the plasma and prolongs the half-life of IGFs and works to either inhibit of stimulate the growth promoting effects while also promoting cell migration and has a length of 259 amino acids and a weight of approximately 28 kDa. Current studies are being done on several diseases and disorders relating to this gene including protein-energy malnutrition, pre-eclampsia, persistent fetal circulation syndrome, Silver-Russell syndrome, growth hormone deficiency, Ehlers-Danlos syndrome, glucose intolerance, Prader-Willi syndrome, and chronic fatigue syndrome. IGFBP1 has also been shown to have interactions with IGF1, IGF2, MMP26, TF, and ARNT in pathways such as the myometrial relaxation and contraction, HIF-1-alpha transcription factor, IGF regulation, and protein metabolism pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for IGFBP-1 ELISA Kit (KA1872). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Blogs on IGFBP-1.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

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Bioinformatics

Gene Symbol IGFBP1