IGF-I R/IGF1R Antibody (33255) [Phycoerythrin]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R PE-conjugated Monoclonal Antibody (Catalog # FAB391P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 33255
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IGF-I R/IGF1R Antibody (33255) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R
  Glu31-Asn932
  Accession # P08069
• Specificity: Detects human IGF-I R in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha , rhIL-9 R, and rhTGF-beta RII.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGF1R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I R/IGF1R Antibody (33255) [Phycoerythrin]

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-I R/IGF1R Antibody (FAB391P)(6)

Publications using FAB391P

• Jørgensen, SH;Emdal, KB;Pedersen, AK;Axelsen, LN;Kildegaard, HF;Demozay, D;Pedersen, TÅ;Grønborg, M;Slaaby, R;Nielsen, PK;Olsen, JV; Multi-layered proteomics identifies insulin-induced upregulation of the EphA2 receptor via the ERK pathway which is dependent on low IGF1R level Scientific reports 2024-11-21 [PMID: 39572596] (FACS, Human)
• Quantification of IGF-1 Receptor May Be Useful in Diagnosing Polycythemia Vera-Suggestion to Be Added to Be One of the Minor Criterion PLoS ONE, 2016-11-03;11(11):e0165299. 2016-11-03 [PMID: 27812134] (Flow Cytometry, Human)
• Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells. Br J Cancer, 2014-05-22;110(12):2865-73. 2014-05-22 [PMID: 24853185] (Flow Cytometry, Human)
• Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R. PLoS ONE, 2014-01-29;9(1):e84417. 2014-01-29 [PMID: 24489649] (Flow Cytometry, Human)
• Muller E, Dunstheimer D, Klammt J, Friebe D, Kiess W, Kratzsch J, Kruis T, Laue S, Pfaffle R, Wallborn T, Heidemann PH Clinical and functional characterization of a patient carrying a compound heterozygous pericentrin mutation and a heterozygous IGF1 receptor mutation. PLoS ONE, 2012-05-31;7(5):e38220. 2012-05-31 [PMID: 22693602] (Flow Cytometry, Human)
• Raile K, Hille R, Laue S, Schulz A, Pfeifer G, Horn F, Kiess W Insulin-like growth factor I (IGF-I) stimulates proliferation but also increases caspase-3 activity, Annexin-V binding, and DNA-fragmentation in human MG63 osteosarcoma cells: co-activation of pro- and anti-apoptotic pathways by IGF-I. Horm. Metab. Res., 2003-11-01;35(11):786-93. 2003-11-01 [PMID: 14710359] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IGF1R
• Uniprot:
  • P08069()