IGF-I/IGF-1 Antibody [Unconjugated]

Images

 
IGF-I/IGF-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. ...read more
Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

IGF-I/IGF-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IGF-I/IGF-1
Gl33-Ala102
Accession # Q8CAR0
Specificity
Detects mouse IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGF-I/IGF-1 is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGF1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2-1.0 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF791 in the following applications:

Publications
Read Publications using
AF791 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I/IGF-1 Antibody [Unconjugated]

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1067
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
AF1067
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF1360
Species: Mu
Applications: WB, IHC
NBP2-68008
Species: Hu
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP2-12793
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
MAB391
Species: Hu, Mu
Applications: WB, Flow, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
AF1445
Species: Mu, Rt
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-37447
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, IF
NBP1-80549
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
7270-IL/CF
Species: Hu
AF791
Species: Mu
Applications: WB, IHC, B/N

Publications for IGF-I/IGF-1 Antibody (AF791)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, In Vivo, Neutralization, Western Blot.


Filter By Application
ICC
(3)
IHC
(3)
IHC-Fr
(2)
In Vivo
(1)
Neutralization
(4)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(14)
Rat
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF791 Applications Species
M Inoue, T Ono, Y Kameo, F Sasaki, T Ono, T Adachi, T Nakashima Forceful mastication activates osteocytes and builds a stout jawbone Sci Rep, 2019;9(1):4404. 2019 [PMID: 30890758] (Neutralization, Mouse) Neutralization Mouse
V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019;10(1):518. 2019 [PMID: 30705270] (IHC, Mouse) IHC Mouse
LE Hillers, JV D'Amato, T Chamberlin, G Paderta, LM Arendt Obesity-Activated Adipose-Derived Stromal Cells Promote Breast Cancer Growth and Invasion Neoplasia, 2018;20(11):1161-1174. 2018 [PMID: 30317122] (Western Blot, Mouse) Western Blot Mouse
JW Park, IY Kim, JW Choi, HJ Lim, JH Shin, YN Kim, SH Lee, Y Son, M Sohn, JK Woo, JH Jeong, C Lee, YS Bae, JK Seong AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development Mol. Cancer Res., 2018;0(0):. 2018 [PMID: 29724814] (IHC, Mouse) IHC Mouse
T Gao, N Bogdanova, S Ghauri, G Zhang, J Lin, K Sheikh Systemic IGF-1 gene delivery by rAAV9 improves spontaneous autoimmune peripheral polyneuropathy (SAPP) Sci Rep, 2018;8(1):5408. 2018 [PMID: 29615658] (Western Blot, Human) Western Blot Human
Z Zuo, F Qi, J Yang, X Wang, Y Wu, Y Wen, Q Yuan, J Zou, K Guo, ZB Yao Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain Neurobiol. Dis, 2017;101(0):27-39. 2017 [PMID: 28189498] (IHC, Mouse) IHC Mouse
K Kimura, T Terasaka, N Iwata, T Katsuyama, M Komatsubar, R Nagao, K Inagaki, F Otsuka Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein J Clin Med, 2017;6(1):. 2017 [PMID: 28067796] (Neutralization, Mouse) Neutralization Mouse
C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism Sci Rep, 2016;6(0):19404. 2016 [PMID: 26762853] (Neutralization, Mouse) Neutralization Mouse
Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling. J Neurosci, 2014;34(38):12788-800. 2014 [PMID: 25232115] (In Vivo, Rat) In Vivo Rat
Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength. Am J Physiol Endocrinol Metab, 2014;306(8):E965-74. 2014 [PMID: 24569593] (Western Blot, Mouse) Western Blot Mouse
Show All 16 Publications.

Review for IGF-I/IGF-1 Antibody (AF791) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF791:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23407451
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23407451

Details

Detection NotesSee PMID 23407451

Comments

CommentsPublished: See PMID 23407451Independent review from antibodyresource.com1:500 dilution detected 0.5 ng recombinant IGF-1.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IGF-I/IGF-1 Antibody (AF791) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF791

Additional IGF-I/IGF-1 Products

Bioinformatics Tool for IGF-I/IGF-1 Antibody (AF791)

Discover related pathways, diseases and genes to IGF-I/IGF-1 Antibody (AF791). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IGF-I/IGF-1 Antibody (AF791)

Discover more about diseases related to IGF-I/IGF-1 Antibody (AF791).
 

Pathways for IGF-I/IGF-1 Antibody (AF791)

View related products by pathway.

PTMs for IGF-I/IGF-1 Antibody (AF791)

Learn more about PTMs related to IGF-I/IGF-1 Antibody (AF791).

Blogs on IGF-I/IGF-1

There are no specific blogs for IGF-I/IGF-1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
01/09/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol IGF1
Uniprot