![Immunohistochemistry: Rabbit anti-Human IgA1 Secondary Antibody (RM124) [Unconjugated] [NBP2-62024] - Rabbit anti- IgA1 Secondary Antibody (124) [Biotin] [NBP2-62024B] - Immunohistochemistry of Human Tonsil Tissue using Anti-Human IgA1 antibody NBP2-62024B. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize2/Rabbit-anti-Human-IgA1-Secondary-Antibody-(RM124)-[Unconjugated]-Immunohistochemistry-NBP2-62024-img0002.jpg "Immunohistochemistry: Rabbit anti-Human IgA1 Secondary Antibody (RM124) [Unconjugated] [NBP2-62024] - Rabbit anti- IgA1 Secondary Antibody (124) [Biotin] [NBP2-62024B] - Immunohistochemistry of Human Tonsil Tissue using Anti-Human IgA1 antibody NBP2-62024B. Image using the Biotin form of this antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clone | RM124 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS with 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
Diseases for IgA1 Antibody (NBP2-62024)Discover more about diseases related to IgA1 Antibody (NBP2-62024).
| Pathways for IgA1 Antibody (NBP2-62024)View related products by pathway.
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Research Areas for IgA1 Antibody (NBP2-62024)Find related products by research area.
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| Gene Symbol | IGHA1 |
![ELISA: Rabbit anti-Human IgA1 Secondary Antibody (RM124) [Unconjugated] [NBP2-62024] - Rabbit anti-Human IgA1 Secondary Antibody (124) [Biotin] [NBP2-62024B] - Sandwich ELISA using RM124 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( kappa + lambda) antibody NBP2-62024B as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize2/Rabbit-anti-Human-IgA1-Secondary-Antibody-(RM124)-[Unconjugated]-ELISA-NBP2-62024-img0001.jpg "ELISA: Rabbit anti-Human IgA1 Secondary Antibody (RM124) [Unconjugated] [NBP2-62024] - Rabbit anti-Human IgA1 Secondary Antibody (124) [Biotin] [NBP2-62024B] - Sandwich ELISA using RM124 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( kappa + lambda) antibody NBP2-62024B as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin. Image using the Biotin form of this antibody.")
![ELISA: Rabbit anti-Human IgA1 Secondary Antibody (RM124) [Unconjugated] [NBP2-62024] - Rabbit anti- IgA1 Secondary Antibody (124) [Biotin] [NBP2-62024B] - ELISA of human immunoglobulins shows NBP2-62024B reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of NBP2-62024B was used as the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondary antibody. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize2/Rabbit-anti-Human-IgA1-Secondary-Antibody-(RM124)-[Unconjugated]-ELISA-NBP2-62024-img0003.jpg "ELISA: Rabbit anti-Human IgA1 Secondary Antibody (RM124) [Unconjugated] [NBP2-62024] - Rabbit anti- IgA1 Secondary Antibody (124) [Biotin] [NBP2-62024B] - ELISA of human immunoglobulins shows NBP2-62024B reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of NBP2-62024B was used as the primary antibody. An alkaline phosphataseconjugated anti-rabbit IgG as the secondary antibody. Image using the Biotin form of this antibody.")
![ELISA: Rabbit anti-Human IgA1 Secondary Antibody (RM124) [Unconjugated] [NBP2-62024] - Rabbit anti- IgA1 Secondary Antibody (124) [Biotin] [NBP2-62024B] - A titer ELISA using NBP2-62024B. The plate was coated with different amounts of human IgA1. A serial dilution of NBP2-62024B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize2/Rabbit-anti-Human-IgA1-Secondary-Antibody-(RM124)-[Unconjugated]-ELISA-NBP2-62024-img0004.jpg "ELISA: Rabbit anti-Human IgA1 Secondary Antibody (RM124) [Unconjugated] [NBP2-62024] - Rabbit anti- IgA1 Secondary Antibody (124) [Biotin] [NBP2-62024B] - A titer ELISA using NBP2-62024B. The plate was coated with different amounts of human IgA1. A serial dilution of NBP2-62024B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Image using the Biotin form of this antibody.")
![Immunocytochemistry/Immunofluorescence: ATP6V0A1 Antibody [NBP1-89342] - Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89342-img0006.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0011.jpg)
![Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize2/ATP6V0A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89342-img0010.jpg)
![Western Blot: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.](http://images.novusbio.com/fullsize2/RFC2 Antibody (2B2)-Western Blot-NBP2-45570-img0013.jpg)
![Immunohistochemistry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(2B2)-Immunohistochemistry-NBP2-45570-img0012.jpg)
![Flow Cytometry: RFC2 Antibody (2B2) [NBP2-45570] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by RFC2 antibody.](http://images.novusbio.com/fullsize2/RFC2-Antibody-(2B2)-Flow-Cytometry-NBP2-45570-img0001.jpg)
![Western Blot: RFC4 Antibody [NBP2-48927] - Analysis using Anti-RFC4 antibody NBP2-48927 (A) shows similar pattern to independent antibody NBP2-49283 (B).](http://images.novusbio.com/fullsize2/RFC4 Antibody-Western Blot-NBP2-48927-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP2-48927] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/RFC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48927-img0008.jpg)
![Immunohistochemistry-Paraffin: RFC4 Antibody [NBP2-48927] - Staining in human testis and kidney tissues using anti-RFC4 antibody. Corresponding RFC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/RFC4-Antibody-Immunohistochemistry-Paraffin-NBP2-48927-img0007.jpg)
![Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Western-Blot-NBP1-84550-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0002.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunocytochemistry-Immunofluorescence-NB100-524-img0008.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Western Blot: FCAR/CD89 Antibody [NBP1-88102] - Analysis in control (vector only transfected HEK293T lysate) and FCAR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/FCAR-CD89 Antibody-Western Blot-NBP1-88102-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: FCAR/CD89 Antibody [NBP1-88102] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/FCAR-CD89-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88102-img0012.jpg)
![Immunohistochemistry-Paraffin: FCAR/CD89 Antibody [NBP1-88102] - Staining of human lymph node shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.](http://images.novusbio.com/fullsize2/FCAR-CD89-Antibody-Immunohistochemistry-Paraffin-NBP1-88102-img0010.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![SDS-Page: Recombinant Human IgA1 Protein [H00003493-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images2/Recombinant Human IgA1 Protein-SDS-Page-H00003493-P02-img0001.jpg "SDS-Page: Recombinant Human IgA1 Protein [H00003493-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
