IgA1 Antibody (RM124)

Immunohistochemistry: IgA1 Antibody (RM124) [Unconjugated] [NBP2-62024] - Immunohistochemistry of Human Tonsil Tissue using NBP2-62024.

ELISA: IgA1 Antibody (RM124) - Azide and BSA Free [NBP2-62024] - ELISA of human immunoglobulins shows RM124 only reacts to human IgA1. The clone very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA: IgA1 Antibody (RM124) - Azide and BSA Free [NBP2-62024] - A titer ELISA using RM124. The plate was coated with different amounts of human IgA1. A serial dilution of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Sandwich ELISA: IgA1 Antibody (RM124) - Azide and BSA Free [NBP2-62024] - Sandwich ELISA using RM124 as the capture antibody (100ng/well), and NBP3-18532 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

• Reactivity: Hu
• Applications: ELISA, ICC/IF, IHC, ELISA
• Clone: RM124
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IgA1 Antibody (RM124) Summary

• Description: Novus Biologicals Rabbit IgA1 Antibody (RM124) (NBP2-62024) is a recombinant monoclonal antibody validated for use in IHC, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human IgA
• Specificity: This antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGHA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 0.1 - 1ug/ml
  • Sandwich ELISA Capture 50 - 200ng/well
  • Sandwich ELISA Detection 0.05 - 0.2ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IgA1 Antibody (RM124)

• Arc-1
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDHE
• cell-CAM 120/80
• ECAD
• E-cadherin
• Epithelial cadherin
• LCAM
• UVOE-Cadherin
• uvomorulin

Background

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against localinfection and to prevent access of foreign antigens to the general immunologic syste

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHA1