IgA Antibody (RM220)

Western Blot: IgA Antibody (RM220) [Unconjugated] [NBP2-62011] - Western blot of nonreduced(-) and reduced(+) Mouse IgA, using NBP2-62011. This antibody reacts to nonreduced IgA.

ELISA: IgA Antibody (RM220) [NBP2-62011] - ELISA of Mouse immunoglobulins shows RM220 reacts to Mouse IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.

ELISA: IgA Antibody (RM220) [Unconjugated] [NBP2-62011] - A titer ELISA of Mouse IgA. The plate was coated with different amount of Mouse IgA. A serial dilution of NBP2-62011 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.

• Reactivity: Mu
• Applications: WB, ELISA
• Clone: RM220
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IgA Antibody (RM220) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse IgA
• Specificity: This antibody specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGHA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.005 ug/ml - 0.2 ug/ml
  • Western Blot 0.5 ug/ml-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IgA Antibody (RM220)

• IgA
• Immunoglobulin A

Background

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. The most common feature of plasmacytomas, and certain non Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non Hodgkin's lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHA1