IgA Antibody (JM10-42)

Western Blot: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of IgA on human plasma lysates using anti-IgA antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: IgA Antibody (JM10-42) [NBP2-66901] - Staining IgA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IgA Antibody (JM10-42) [NBP2-66901] - Analysis of paraffin-embedded human spleen tissue using anti-IgA antibody. Counter stained with hematoxylin.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: JM10-42
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IgA Antibody (JM10-42) Summary

• Description: Novus Biologicals Rabbit IgA Antibody (JM10-42) (NBP2-66901) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant full length protein of Human IgA aa 1-353 / 353. (SwissProt: P01876 Human; SwissProt: P01877 Human)
• Localization: Cell membrane, Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGHA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IgA Antibody (JM10-42)

• IgA
• Immunoglobulin A

Background

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. The most common feature of plasmacytomas, and certain non Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non Hodgkin's lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHA1