IFT122 Overexpression Lysate

Images

 
Western Blot: IFT122 Overexpression Lysate (Adult Normal) [NBL1-11856] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFT122.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IFT122 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200119 and based on accession number NM_052990. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 4, mRNA.
Gene
IFT122

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
129.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IFT122 Overexpression Lysate

  • intraflagellar transport 122 homolog (Chlamydomonas)
  • intraflagellar transport protein 122 homolog
  • SPGWD repeat-containing protein 140
  • WD repeat domain 10
  • WD repeat-containing protein 10
  • WDR10WDR10p
  • WDR140CED

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Transcript Variant: This variant (4) lacks 4 in-frame exons in the 5' region, as compared to variant 1. The resulting protein (isoform 4) is shorter than isoform 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IFT122