IFN-kappa Antibody [Unconjugated]

Images

 
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ...read more
Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and EL‑4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for IFN‑ kappa at approximately 40 kDa ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IFN-kappa Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IFN-kappa
Leu22-Lys199 (Cys174Tyr)
Accession # Q7TSL0
Specificity
Detects mouse IFN-kappa in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
IFNK
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
Publications
Read Publication using
AF5206 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-kappa Antibody [Unconjugated]

  • IFNK
  • IFNkappa
  • IFN-kappa

Background

IFN-kappa (Interferon kappa) is a secreted, nonglycosylated polypeptide that belongs to the IFN-kappa subclass of the type I interferon family of cytokines. It is expressed by macrophages in mice, and monocytes, dendritic cells and keratinocytes in human. IFN-kappa induces TNF-alpha, IL-10 and MCP-1 production by monocytes. Mouse IFN-kappa precursor is 199 amino acids (aa) in length. It contains a 21 aa signal sequence, plus a 178 aa mature region. There are two intrachain disulfide bonds plus a fifth unpaired cysteine. Mature mouse IFN-kappa (aa 22‑199) shares 68% and 30% aa identity with rat and human IFN-kappa, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFN-kappa Antibody (AF5206)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC.


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(1)
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Video Protocols

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Bioinformatics

Gene Symbol IFNK
Uniprot