IFITM3/Fragilis Antibody

Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

IFITM3/Fragilis was detected in immersion fixed frozen sections of embryonic mouse ovary (E13.5) using 10 µg/mL Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IFITM3/Fragilis Antibody Summary

• Immunogen: E. coli-derived recombinant mouse IFITM3/Fragilis
  Asn2-His57
  Accession # Q9CQW9
• Specificity: Detects mouse IFITM3/Fragilis in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IFITM3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFITM3
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFITM3/Fragilis Antibody

• 1-8U
• Fragilis
• IFITM3
• interferon induced transmembrane protein 3 (1-8U)
• interferon-induced transmembrane protein 3
• Interferon-inducible protein 1-8U
• IP15
• mil-1

Background

Mouse IFITM3/CD225 (also Fragilis) is a 137 amino acids (aa) 2-transmembrane protein that belongs to the Fragilis family of interferon-inducible genes. It contains two terminal extracellular domains (aa 1-57 and 131-137) and one cytoplasmic region that potentially allows for multiple phosphorylations. The N-terminal extracellular region of mouse IFITM3 shares 54%, 91%, and 58% amino acid sequence identity to the equivalent region in bovine, rat and human IFITM3, respectively. IFITM3 seems to be involved in primordial germ cell clustering and regionalization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFITM3/Fragilis Antibody (AF3377)(5)

Publications using AF3377

• J Zhang, M Zhang, D Acampora, M Vojtek, D Yuan, A Simeone, I Chambers OTX2 restricts entry to the mouse germline Nature, 2018;0(0):. 2018-01-01 [PMID: 30283136] (IHC-P, Mouse)
• SK Kuss-Duerk, J Wang, I Mena, K White, G Metreveli, R Sakthivel, MA Mata, R Muñoz-More, X Chen, F Krammer, MS Diamond, ZJ Chen, A García-Sas, BMA Fontoura Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication PLoS Pathog., 2017;13(9):e1006635. 2017-01-01 [PMID: 28953980] (Western Blot, Mouse)
• Y Fu, Z Zhou, H Wang, P Gong, R Guo, J Wang, X Lu, F Qi, L Liu IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells FEBS Open Bio, 2017;7(8):1102-1110. 2017-01-01 [PMID: 28781951] (Western Blot, Human)
• Bailey , Charles, Kondur , Hema R, Huang , I-Chueh, Farzan , Michael Interferon-induced transmembrane protein 3 is a type II transmembrane protein. J Biol Chem, 2013;288(45):32184-93. 2013-01-01 [PMID: 24067232] (Western Blot, Mouse)
• Bailey , Charles, Huang , I-Chueh, Kam , Christin, Farzan , Michael Ifitm3 limits the severity of acute influenza in mice. PLoS Pathog, 2012;8(9):e1002909. 2012-01-01 [PMID: 22969429] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IFITM3
• Uniprot:
  • Q9CQW9()