IFITM3/Fragilis Antibody

Images

 
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
IFITM3/Fragilis was detected in immersion fixed frozen sections of embryonic mouse ovary (E13.5) using 10 µg/mL Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) overnight ...read more
Viral replication is required for influenza virus to activate mTORC1 independently of NS1.(A, B) Vero cells were infected with (A) WSN or (B) PR8 wild-type or mutant viruses lacking NS1 at MOI of 2 PFU/cell for 6 h. ...read more
Both interferons and gp130-mediated cytokines induce Ifitm3 expression.Ifitm3 protein expression can also be induced by gp130-family cytokines in cells expressing appropriate receptors. The Ifitm3 promoter alone among ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

IFITM3/Fragilis Antibody Summary

Immunogen
E. coli-derived recombinant mouse IFITM3/Fragilis
Asn2-His57
Accession # Q9CQW9
Specificity
Detects mouse IFITM3/Fragilis in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IFITM3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFITM3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3377 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFITM3/Fragilis Antibody

  • 1-8U
  • Fragilis
  • IFITM3
  • interferon induced transmembrane protein 3 (1-8U)
  • interferon-induced transmembrane protein 3
  • Interferon-inducible protein 1-8U
  • IP15
  • mil-1

Background

Mouse IFITM3/CD225 (also Fragilis) is a 137 amino acids (aa) 2-transmembrane protein that belongs to the Fragilis family of interferon-inducible genes. It contains two terminal extracellular domains (aa 1-57 and 131-137) and one cytoplasmic region that potentially allows for multiple phosphorylations. The N-terminal extracellular region of mouse IFITM3 shares 54%, 91%, and 58% amino acid sequence identity to the equivalent region in bovine, rat and human IFITM3, respectively. IFITM3 seems to be involved in primordial germ cell clustering and regionalization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89345
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
H00010581-M14
Species: Hu
Applications: ELISA, S-ELISA, WB
NBP2-75930
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AF1759
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
AF808
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
AF2030
Species: Hu
Applications: IHC, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP2-01325
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-35339
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NBP1-32905
Species: Bv, Hu, Pm, Rb
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-03971
Species: Bv, Ca, Ch, ChHa, Eq, Hu, Mu, Po, Pm, Rb, Rt
Applications: IHC, IHC-P, WB
H00002537-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NB100-594
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
AF8691
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
AF2566
Species: Mu
Applications: IHC, WB
NB600-235
Species: Hu, Mu, Po
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB100-56104
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
DDX0390P-100
Species: Hu, Mu
Applications: B/N, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vivo
NB110-1244
Species: Hu, Mu, Pm
Applications: Flow, IHC, IHC-P, PEP-ELISA

Publications for IFITM3/Fragilis Antibody (AF3377)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC-P
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF3377 Applications Species
Bullard WL, Kara M, Gay LA et al. Identification of murine gammaherpesvirus 68 miRNA-mRNA hybrids reveals miRNA target conservation among gammaherpesviruses including host translation and protein modification machinery PLoS Pathog. 8/1/2019 [PMID: 31393953]
J Zhang, M Zhang, D Acampora, M Vojtek, D Yuan, A Simeone, I Chambers OTX2 restricts entry to the mouse germline Nature, 2018-10-03;0(0):. 2018-10-03 [PMID: 30283136] (IHC-P, Mouse) IHC-P Mouse
SK Kuss-Duerk, J Wang, I Mena, K White, G Metreveli, R Sakthivel, MA Mata, R Muñoz-More, X Chen, F Krammer, MS Diamond, ZJ Chen, A García-Sas, BMA Fontoura Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication PLoS Pathog., 2017-09-27;13(9):e1006635. 2017-09-27 [PMID: 28953980] (Western Blot, Mouse) Western Blot Mouse
Y Fu, Z Zhou, H Wang, P Gong, R Guo, J Wang, X Lu, F Qi, L Liu IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells FEBS Open Bio, 2017-06-29;7(8):1102-1110. 2017-06-29 [PMID: 28781951] (ICC, Western Blot, Human) ICC, Western Blot Human
Bailey , Charles, Kondur , Hema R, Huang , I-Chueh, Farzan , Michael Interferon-induced transmembrane protein 3 is a type II transmembrane protein. J Biol Chem, 2013-09-25;288(45):32184-93. 2013-09-25 [PMID: 24067232] (Western Blot, Mouse) Western Blot Mouse
Bailey , Charles, Huang , I-Chueh, Kam , Christin, Farzan , Michael Ifitm3 limits the severity of acute influenza in mice. PLoS Pathog, 2012-09-06;8(9):e1002909. 2012-09-06 [PMID: 22969429] (Western Blot, Mouse) Western Blot Mouse

Reviews for IFITM3/Fragilis Antibody (AF3377) (0)

There are no reviews for IFITM3/Fragilis Antibody (AF3377). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IFITM3/Fragilis Antibody (AF3377) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IFITM3/Fragilis Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol IFITM3
Uniprot