HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3377-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89401-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27788-50ul | |||
NL001) and counterstained with DAPI (blue). View our protocol for 
![<b>Biological Strategies Validation. </b>Both interferons and gp130-mediated cytokines induce Ifitm3 expression.Ifitm3 protein expression can also be induced by gp130-family cytokines in cells expressing appropriate receptors. The Ifitm3 promoter alone among murine Ifitm promoters is interferon responsive. (A) Expression of Ifitm3 was determined by western blot in NIH 3T3 and RAW 264.7 cells treated for 2 days with the indicated cytokines. IFN lambda 2 failed to induce Ifitm3 expression at similar concentrations, likely due to lack of the type III IFN receptor. The gp130-family cytokine oncostatin M (OSM) was a potent inducer of Ifitm3 expression in 3T3 cells whereas RAW264.7 cells were responsive to IL-6, but not OSM. (B) Surface expression of the OSM receptor, IL-6 receptor, and gp130 by NIH 3T3 and RAW264.7 cells was determined by flow cytometry. Solid blue lines correspond to the fluorescence signal of the indicated receptor-binding antibodies and red lines show fluorescence of the isotype control. The responsiveness of 3T3 and RAW cells to OSM and IL-6 respectively (as shown in panel A) correlates with their expression of their corresponding receptors. (C) Activities of the six murine Ifitm promoters were assayed in mock-treated, IFN alpha 2-treated, and IFN gamma -treated NIH 3T3 cells transfected with plasmids encoding luciferase under the control of the indicated promoters. Ifitm1, 2, and 6 promoters appear constitutively active, and in contrast to human orthologs, insensitive to interferon stimulation. The Ifitm5 promoter shows almost no activity under any condition, consistent with its tissue-restricted expression in vivo[15]. The Ifitm3 promoter alone is interferon responsive (4.4-fold induction with IFN alpha 2; 2.2-fold with IFN gamma ). Bars show mean of triplicates ± SEM. Asterisk indicates p<0.0001 (two-factor ANOVA Bonferroni post test). (D) Cytokine regulation of Ifitm1, 2, and 3 was confirmed at the mRNA level by qRT-PCR in both 3T3 and RAW cells. Bars depict fold upregulation of the indicated transcripts following a](http://images.novusbio.com/fullsize/af3377_mouse-ifitm3-fragilis-affinity-purified-polyclonal-ab-41202412401228.jpg)
![Western Blot: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Western blot analysis of IFITM3/Fragilis on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Western-Blot-NBP2-75533-img0008.jpg "Western Blot: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Western blot analysis of IFITM3/Fragilis on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunocytochemistry-Immunofluorescence-NBP2-75533-img0004.jpg)
![Immunohistochemistry-Paraffin: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of paraffin-embedded human colon cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunohistochemistry-Paraffin-NBP2-75533-img0006.jpg)
![Flow Cytometry: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Flow-Cytometry-NBP2-75533-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunocytochemistry-Immunofluorescence-NBP2-75533-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Staining Fragilis in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunocytochemistry-Immunofluorescence-NBP2-75533-img0003.jpg)
![Immunohistochemistry-Paraffin: IFITM3/Fragilis Antibody (JU73-02) [NBP2-75533] - Analysis of paraffin-embedded human liver cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-JU73-02-Immunohistochemistry-Paraffin-NBP2-75533-img0005.jpg)
![Western Blot: IFITM3/Fragilis Antibody [NBP1-89401] - Analysis in human cell lines SK-MEL-30 and Caco-2 using anti-IFITM3 antibody. Corresponding IFITM3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-Western-Blot-NBP1-89401-img0011.jpg "Western Blot: IFITM3/Fragilis Antibody [NBP1-89401] - Analysis in human cell lines SK-MEL-30 and Caco-2 using anti-IFITM3 antibody. Corresponding IFITM3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.")
![Western Blot: IFITM3/Fragilis Antibody [NB600-1534] - Analysis of Fragilis in mouse ES lysate in the (1) absence and (2) presence of immunizing peptide and in mouse NIH3T3 lysate in the (3) absence and (4) presence of immunizing peptide using Fragilis antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-Western-Blot-NB600-1534-img0002.jpg "Western Blot: IFITM3/Fragilis Antibody [NB600-1534] - Analysis of Fragilis in mouse ES lysate in the (1) absence and (2) presence of immunizing peptide and in mouse NIH3T3 lysate in the (3) absence and (4) presence of immunizing peptide using Fragilis antibody at 1 ug/ml.")
![Simple Western: IFITM3/Fragilis Antibody [NB600-1534] - Simple Western lane view shows a specific band for Fragilis in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IFITM3-Fragilis-Antibody-Simple-Western-NB600-1534-img0003.jpg)
![Western Blot: IFITM3/Fragilis Antibody (SR3629) [NBP3-27788] - Western blot analysis of IFITM3/Fragilis expression in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-27788_rabbit-ifitm3-fragilis-mab-sr3629-75202410133498.jpg "Western Blot: IFITM3/Fragilis Antibody (SR3629) [NBP3-27788] - Western blot analysis of IFITM3/Fragilis expression in HeLa cell lysate.")
![Western Blot: IFITM3 Overexpression Lysate (Adult Normal) [NBP2-06569] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFITM3.](http://images.novusbio.com/fullsize/IFITM3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06569-img0001.jpg "Western Blot: IFITM3 Overexpression Lysate (Adult Normal) [NBP2-06569] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFITM3.")
![ELISA: Human IFITM3/Fragilis ELISA Kit (Colorimetric) [NBP3-38942] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38942_human-ifitm3-fragilis-elisa-kit-colorimetric-211020241528268.png "ELISA: Human IFITM3/Fragilis ELISA Kit (Colorimetric) [NBP3-38942] - Standard Curve Reference")
![ELISA: Human IFITM3/Fragilis - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38943] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38943_human-ifitm3-fragilis-ready-to-use-elisa-kit-color-211020241529114.png "ELISA: Human IFITM3/Fragilis - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38943] - Standard Curve Reference")
