Calponin 2 Antibody (OTI2B5)

Western Blot: Calponin 2 Antibody (2B5) [NBP2-01325] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calponin 2 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Calponin 2 Antibody (2B5) [NBP2-01325] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Calponin 2.

Immunohistochemistry-Paraffin: Calponin 2 Antibody (2B5) [NBP2-01325] - Staining of paraffin-embedded Human tonsil using anti-Calponin 2 mouse monoclonal antibody.

Western Blot: Calponin 2 Antibody (2B5) [NBP2-01325] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calponin 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calponin 2.

Immunohistochemistry-Paraffin: Calponin 2 Antibody (2B5) [NBP2-01325] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Calponin 2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Calponin 2 Antibody (2B5) [NBP2-01325] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Calponin 2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Calponin 2 Antibody (2B5) [NBP2-01325] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Calponin 2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Calponin 2 Antibody (2B5) [NBP2-01325] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Calponin 2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Calponin 2 Antibody (2B5) [NBP2-01325] - Staining of paraffin-embedded Human lymphoma tissue using anti-Calponin 2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI2B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.26 mg/ml

Calponin 2 Antibody (OTI2B5) Summary

• Description: Novus Biologicals Mouse Calponin 2 Antibody (OTI2B5) (NBP2-01325) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Calponin 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CNN2(NP_004359) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 33.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.26 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Calponin 2 Antibody (OTI2B5)

• calponin 2
• Calponin H2, smooth muscle
• calponin-2
• Neutral calponin

Background

The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calponin 2 Antibody (NBP2-01325)(1)

Publication using NBP2-01325

• Paredes F, Williams HC, Quintana RA, San Martin A. Mitochondrial Protein Poldip2 (Polymerase Delta Interacting Protein 2) Controls Vascular Smooth Muscle Differentiated Phenotype by O-Linked GlcNAc (N-Acetylglucosamine) Transferase-Dependent Inhibition of a Ubiquitin Proteasome System Circulation Research 2020-01-03 [PMID: 31656131] (Western Blot)

Bioinformatics

• Gene Symbol: CNN2
• Entrez:
  • 1265(Human)