IFITM1 Antibody - BSA Free

Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining in human epididymis and testis tissues using anti-IFITM1 antibody. Corresponding IFITM1 RNA-seq data are presented for the same tissues.

Western Blot: IFITM1 Antibody [NBP1-89345] - Analysis in human cell line CAPAN-2.

Immunocytochemistry/Immunofluorescence: IFITM1 Antibody [NBP1-89345] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human epididymis shows strong membranous positivity in ependymal cells.

Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.

Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human testis shows no membranous positivity in cells in seminiferous ducts as expected.

Simple Western: IFITM1 Antibody [NBP1-89345] - Simple Western lane view shows a specific band for IFITM1 in 0.2 mg/ml of h. Tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: IFITM1 Antibody [NBP1-89345] - Electropherogram image(s) of corresponding Simple Western lane view. IFITM1 antibody was used at 1:20 dilution on h. Tonsil lysate(s).

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IFITM1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MHKEEHEVAVLGAPPSTILPRSTVINIHSETSVPDH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFITM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in h. Tonsil, separated by Size, antibody dilution of 1:20, apparent MW was 20 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control Peptide:

  IFITM1 Protein (NBP1-89345PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24072182)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for IFITM1 Antibody - BSA Free

• CD225 antigen
• CD225
• CD225Leu-13 antigen
• IFI17
• IFI17LEU13,9-27
• IFITM1
• interferon induced transmembrane protein 1 (9-27)
• Interferon-induced protein 17
• interferon-induced transmembrane protein 1
• Interferon-inducible protein 9-27
• Leu-13

Background

Implicated in the control of cell growth. Component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFITM1 Antibody (NBP1-89345)(3)

Publications using NBP1-89345

• Luo HL, Lee YC, Chang YL et al. MicroRNA-145-5p suppresses cell proliferation, migration, and invasion in upper tract urothelial carcinoma by targeting 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase Journal of cellular biochemistry 2023-07-20 [PMID: 37475541]
• Parra-Herran CE, Yuan L, Nucci MR, Quade BJ. Targeted development of specific biomarkers of endometrial stromal cell differentiation using bioinformatics: the IFITM1 model. Mod Pathol 2014-04-01 [PMID: 24072182]
• Klymiuk I, Kenner L, Adler T et al. In vivo functional requirement of the mouse Ifitm1 gene for germ cell development, interferon mediated immune response and somitogenesis. PLoS One 2012-01-01 [PMID: 23115618]

Bioinformatics

• Gene Symbol: IFITM1