IFITM1 Antibody

Images

 
Western Blot: IFITM1 Antibody [NBP1-89345] - Analysis in human cell line CAPAN-2.
Immunocytochemistry/ Immunofluorescence: IFITM1 Antibody [NBP1-89345] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human epididymis shows high expression.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human testis shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining in human epididymis and testis tissues using anti-IFITM1 antibody. Corresponding IFITM1 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human epididymis shows strong membranous positivity in ependymal cells.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.
Immunohistochemistry-Paraffin: IFITM1 Antibody [NBP1-89345] - Staining of human testis shows no membranous positivity in cells in seminiferous ducts as expected.
Simple Western: IFITM1 Antibody [NBP1-89345] - Simple Western lane view shows a specific band for IFITM1 in 0.2 mg/ml of h. Tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Simple Western: IFITM1 Antibody [NBP1-89345] - Electropherogram image(s) of corresponding Simple Western lane view. IFITM1 antibody was used at 1:20 dilution on h. Tonsil lysate(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

IFITM1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MHKEEHEVAVLGAPPSTILPRSTVINIHSETSVPDH
Specificity
Specificity of human IFITM1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFITM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
IFITM1 Knockout HeLa Cell Lysate
Control Peptide
Publications
Read Publications using NBP1-89345.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24072182)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IFITM1 Antibody

  • CD225 antigen
  • CD225
  • CD225Leu-13 antigen
  • IFI17
  • IFI17LEU13,9-27
  • IFITM1
  • interferon induced transmembrane protein 1 (9-27)
  • Interferon-induced protein 17
  • interferon-induced transmembrane protein 1
  • Interferon-inducible protein 9-27
  • Leu-13

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFITM1 Antibody (NBP1-89345)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IFITM1 Antibody (NBP1-89345) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for IFITM1 Antibody (NBP1-89345)

Discover related pathways, diseases and genes to IFITM1 Antibody (NBP1-89345). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on IFITM1

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Bioinformatics

Gene Symbol IFITM1