IFIT2 Antibody - BSA Free

Western Blot: IFIT2 Antibody [NBP2-15180] - MAPL is required for early steps of immune response to Sendai virus infection. Maplfl/fl and Mapl-/- MEFs were left untreated or infected with Sendai virus (150 HAU/mL) for up to 18 hours and lysates were immunoblotted. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-00151-6), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: IFIT2 Antibody [NBP2-15180] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IFIT2 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Western Blot: IFIT2 Antibody [NBP2-15180] - Analysis of IFIT2 in human K562 lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW lysate probed with 4 ug/ml of IFIT2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Pm, Pm-Or, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

IFIT2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IFIT2 Antibody - BSA Free (NBP2-15180) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-IFIT2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 320-370 from human IFIT2 was used as the immunogen for this antibody.
• Predicted Species: Orangutan (94%), Chimpanzee (94%), Rhesus Macaque (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFIT2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5-10 ug/ml
  • Western Blot 2-5 ug/ml

Reactivity Notes

The IFIT2 amino acid sequence used as immunogen for this antibody is 94% in White-cheeked Gibbon.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for IFIT2 Antibody - BSA Free

• cig42
• G10P2IFI-54K
• GARG-39
• IFI-54
• IFI54IFIT-2
• IFI-54K
• Interferon, alpha-inducible protein (MW 54kD)
• Interferon-induced 54 kDa protein
• interferon-induced protein 54
• interferon-induced protein with tetratricopeptide repeats 2
• ISG-54 K
• ISG54
• ISG-54K
• P54

Background

IFIT2, or IFIT2/SG54, has been identified as a 54kDa protein and an important component of the TLR signaling family and ISG family of proteins induced by Type 1 Interferons. IFIT2 is one of three members of tetraticopeptide repeat (IFIT) gene in mice. IFIT2 knockout mice succumb to intranasal VSV infection exhibiting neurological patterns such as ataxia before death. This is caused by spread from olfactory lobes to other brain regions where VSV titers rise over a hundred-fold in IFIT2 knockout but not in wild-type mice. IFIT1 knockouts and WT mice survive IN injection. Additionally in West Nile Virus infections ISGs and IFIT2 in particular appear to drive the protective IFN Type I responses. IKKe induces STAT1 phosphorylation which drives IFIT expression mediating IFN anti-WNV effects. WNV replicates unabatedly in cells from IKKe-/- or IFIT2-/- mice. STAT1 phosphorylation is greatly diminished in these mice. Antibody to IFIT2 provides a useful probe to further characterize its specific antiviral and IFN mediating role as shown in the aforementioned models.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFIT2 Antibody (NBP2-15180)(1)

Publication using NBP2-15180

• Doiron K, Goyon V, Coyaud E et al. The dynamic interacting landscape of MAPL reveals essential functions for SUMOylation in innate immunity. Sci Rep. 2017-12-01 [PMID: 28273895] (Mouse)

Bioinformatics

• Gene Symbol: IFIT2
• Uniprot:
  • NP_001538.4(Human)