Home » Ifi204 » Ifi204 Antibodies » Ifi204 Antibody

Ifi204 Antibody

 (3 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: Ifi204 Antibody [NBP2-27153] - Analysis of Ifi204 in mouse heart tissue lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: Ifi204 Antibody [NBP2-27153] - Analysis of Ifi204 in mouse heart tissue lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and ...read more
Immunohistochemistry-Paraffin: Ifi204 Antibody [NBP2-27153] - Analysis of Ifi204 in formalin-fixed, paraffin-embedded mouse testis tissue using this antibody at 5 ug/ml.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

100% Guarantee on every product

Ifi204 Antibody Summary

Immunogen
A portion of amino acids 70-120 of mouse Ifi204 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFI204
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 2-4ug/ml~
Publications
Read Publications using
NBP2-27153 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Ifi204 Antibody

  • Ifi16
  • Ifi204 interferon activated gene 204
  • p204

Background

Interferon-activable protein 204 belongs to the HIN-200 family, which contains one DAPIN domain and two HIN-200 domains. It is reported to inhibit transcription of ribosomal RNA and thus acts as a multifunctional regulator of cell proliferation and differentiation. It is reported to act as a transcriptional coactivator of Cbfa1 (Core binding factor alpha-1) and enhances osteoblast differentiation. It enables skeletal muscle and cardiac myocyte differentiation by sequestering Id proteins in the cytosol and promoting their ubiquitination and subsequent degradation. It is also reported that Ifi204 is trancriptionally activated in response to M-CSF and thus involved in macrophage differentiation in myeloid progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AIM2 Antibody
(10M5G5)
NBP2-27354
Western Blot: AIM2 Antibody (10M5G5) [NBP2-27354] - Analysis using AIM2 antibody. Recombinant human AIM2 probed with AIM2 antibody at 2 ug/ml.
Western Blot: AIM2 Antibody (10M5G5) [NBP2-27354] - Western blot analysis of Daudi lysate (35 ug per lane, RIPA buffer) using AIM2 antibody (NBP2-27354) at 0.1ug/ml. Band observed at ~38kDa. (Expected MW of 39.0kDa according to NP_004824.1).
(1 Publication)
Species: Hu
Applications: WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(15 Publications)
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IFN-gamma
[Unconjugated]
285-IF
Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma .
1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
(24 Reviews)
(389 Publications)
Species: Hu
Applications: BA
53BP1 Antibody -
BSA Free
NB100-304
Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.
Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [NB100-304] - Human medulloblastoma (DAOY) and mouse astrocyte (C8D1A) cell lines were exposed for 48 hours to DMSO or 1ug/mL of the DNA damaging agent EPE. Cells were immunostained for 53BP1 (green). The nuclei were counterstained with DAPI (blue). Image from verified customer review.
(14 Reviews)
(567 Publications)
Species: Bt, Bv, Ca, Fi, Gt, Hu, Mu, Pm, Rt
Applications: ChIP, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, KD, KO, WB
MNDA Antibody
(OTI4H6)
NBP2-45907
Immunohistochemistry: MNDA Antibody (OTI4H6) [NBP2-45907] - MNDA Antibody (4H6) [NBP2-45907] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)
Immunohistochemistry: MNDA Antibody (4H6) [NBP2-45907] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: MNDA Antibody (4H6) [NBP2-45907] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Species: Hu
Applications: IHC, IHC-P, WB
RB1 Antibody
(172C1094) - (cleaved
NB100-56598
Knockdown Validated: RB1 Antibody (172C1094) - (cleaved) [NB100-56598] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and RB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human RB-1 Monoclonal Antibody (Catalog # NB100-56598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for RB-1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.
Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.
(2 Publications)
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, KD, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
(3 Reviews)
(33 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
IFN-alpha 1
Antibody
NBP2-75930
Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.
Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IFI202b Antibody
NBP2-27066
Western Blot: IFI202b Antibody [NBP2-27066] - Analysis of Ifi202b in mouse spleen tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
(3 Publications)
Species: Hu, Mu
Applications: WB
ID1 Antibody
[Unconjugated]
AF4377
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 20 μg of cytoplasmic (Cyto) and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for ID1 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ID1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into neural progenitor cells (upper panel), using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(3 Publications)
Species: Hu, Mu
Applications: ICC, Simple Western, WB
PYHIN1 Antibody
NBP2-13835
Immunohistochemistry-Paraffin: PYHIN1 Antibody [NBP2-13835] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: PYHIN1 Antibody [NBP2-13835] - Staining of human rectum shows moderate cytoplasmic/ nuclear positivity in lymphoid cells.
Immunohistochemistry-Paraffin: PYHIN1 Antibody [NBP2-13835] - Staining of human testis shows weak nuclear positivity in a small subset of cells in seminiferous ducts.
Species: Hu
Applications: IHC, IHC-P
PAK3 [p Ser144, p
Ser141, p Ser139] Ant ...
NBP2-67484
Flow Cytometry: PAK3 [p Ser144, p Ser141, p Ser139] Antibody (SU03-70) [NBP2-67484] - Analysis of NIH/3T3 cells with Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used
Immunocytochemistry/Immunofluorescence: PAK3 [p Ser144, p Ser141, p Ser139] Antibody (SU03-70) [NBP2-67484] - Staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: PAK3 [p Ser144, p Ser141, p Ser139] Antibody (SU03-70) [NBP2-67484] - Staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
IL-3 [Unconjugated]
203-IL/CF
1 μg/lane of Recombinant Human IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-3 (Catalog #203‑IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.02‑0.1 ng/mL.
(169 Publications)
Species: Hu
Applications: BA
NKX2.5 Antibody
AF2444
NKX2.5 Antibody
(2 Reviews)
(4 Publications)
Species: Hu
Applications: IHC, WB
BMP-2
[Unconjugated]
355-BM/CF
Recombinant human/mouse/rat BMP-2 (355-BM/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 40-200 ng/mL.
(168 Publications)
Species: Hu, Mu, Rt
Applications: BA
IRF3 Antibody
(SD2062)
NBP2-67741
Immunocytochemistry/Immunofluorescence: IRF3 Antibody (SD2062) [NBP2-67741] - Staining IRF3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: IRF3 Antibody (SD2062) [NBP2-67741] - Staining IRF3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IRF3 Antibody (SD2062) [NBP2-67741] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-IRF3 antibody. Counter stained with hematoxylin.
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TCEAL1 Antibody
(OTI2G10)
NBP2-46085
Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Species: Hu
Applications: IHC, WB
DIO3 Antibody -
BSA Free
NBP1-05767
Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.
Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.
(2 Reviews)
(14 Publications)
Species: Ha, Hu, Mu, Rb, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
D4S234E Antibody
(1C3)
H00027065-M01
Sandwich ELISA: D4S234E Antibody (1C3) [H00027065-M01] - Detection limit for recombinant GST tagged D4S234E is approximately 0.1ng/ml as a capture antibody.
Western Blot: D4S234E Antibody (1C3) [H00027065-M01] - D4S234E monoclonal antibody (M01), clone 1C3. Analysis of D4S234E expression in PC-12.
Western Blot: D4S234E Antibody (1C3) [H00027065-M01] - D4S234E monoclonal antibody (M01), clone 1C3. Analysis of D4S234E expression in rat brain.
(1 Publication)
Species: Hu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
Ifi204 Antibody
NBP2-27153
Ifi204 Antibody
Species: Mu
Applications: WB, IHC, IHC-P
Supplier Logo

Publications for Ifi204 Antibody (NBP2-27153)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for Ifi204 Antibody (NBP2-27153) (0)

There are no reviews for Ifi204 Antibody (NBP2-27153). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ifi204 Antibody (NBP2-27153) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Ifi204 Products

Array NBP2-27153

Bioinformatics Tool for Ifi204 Antibody (NBP2-27153)

Discover related pathways, diseases and genes to Ifi204 Antibody (NBP2-27153). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ifi204 Antibody (NBP2-27153)

Discover more about diseases related to Ifi204 Antibody (NBP2-27153).
 

Pathways for Ifi204 Antibody (NBP2-27153)

View related products by pathway.

PTMs for Ifi204 Antibody (NBP2-27153)

Learn more about PTMs related to Ifi204 Antibody (NBP2-27153).

Blogs on Ifi204

There are no specific blogs for Ifi204, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ifi204 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol IFI204
Uniprot