IFI16 Antibody - BSA Free

Western Blot: IFI16 Antibody [NBP3-18336] - Western Blot Validation in Rat Testis. Loading: 15 ug of lysate per lane. Antibodies: IFI16 NBP3-18336, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Western Blot: IFI16 Antibody [NBP3-18336] - WB Validation in Human and Rat Cell Lines. Loading: 15 ug of lysate per lane. Antibodies: IFI16 NBP3-18336, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.

Western Blot: IFI16 Antibody [NBP3-18336] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: IFI16 NBP3-18336, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunohistochemistry-Paraffin: IFI16 Antibody [NBP3-18336] - Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (NBP3-18336) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry-Paraffin: IFI16 Antibody [NBP3-18336] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (NBP3-18336) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: IFI16 Antibody - BSA Free [NBP3-18336] - Validation of IFI16 in Human Molt4 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry: IFI16 Antibody - BSA Free [NBP3-18336] - Validation of IFI16 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IFI16 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IFI16 Antibody - BSA Free (NBP3-18336) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of human IFI16. The immunogen is located within the last 50 amino acids of IFI16.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFI16
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 2 ug/mL
• Control Peptide:

  IFI16 Protein (NBP3-18336PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IFI16 Antibody - BSA Free

• ifi-16
• IFNGIP1gamma-interferon-inducible protein 16
• interferon, gamma-inducible protein 16
• interferon-gamma induced protein IFI 16
• Interferon-inducible myeloid differentiation transcriptional activator
• MGC9466
• PYHIN2

Background

IFI16 encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFI16