Iduronate 2-Sulfatase/IDS Antibody (OTI4G2)

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Iduronate 2 sulfatase monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Iduronate 2 sulfatase.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Human tonsil.

Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Iduronate 2 sulfatase antibody, and then analyzed by flow cytometry.

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Iduronate 2 sulfatase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Iduronate 2 sulfatase.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human Kidney tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human liver tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human lymphoma tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Staining of paraffin-embedded Human prostate tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Carcinoma of Human liver tissue.

Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Analysis of Hela cells, using anti-Iduronate 2 sulfatase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (4G2) [NBP2-01761] - Analysis of Jurkat cells, using anti-Iduronate 2 sulfatase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI4G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.71 mg/ml

Iduronate 2-Sulfatase/IDS Antibody (OTI4G2) Summary

• Description: Novus Biologicals Mouse Iduronate 2-Sulfatase/IDS Antibody (OTI4G2) (NBP2-01761) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Iduronate 2-Sulfatase/IDS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human IDS(NP_000193) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 59.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.71 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Iduronate 2-Sulfatase/IDS Antibody (OTI4G2)

• Alpha-L-iduronate sulfate sulfatase
• EC 3.1.6.13
• IDS
• iduronate 2-sulfatase 14 kDa chain
• iduronate 2-sulfatase 42 kDa chain
• iduronate 2-sulfatase
• idursulfase
• MPS2
• S
• SIDS

Background

Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence similarity with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Iduronate 2-Sulfatase/IDS Antibody (NBP2-01761)(1)

Publication using NBP2-01761

• Bellesso S, Salvalaio M, Lualdi S et al. FGF signaling deregulation is associated with early developmental skeletal defects in animal models for mucopolysaccharidosis type II (MPSII) Hum. Mol. Genet. 2018-04-10 [PMID: 29648648] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: IDS
• Entrez:
  • 3423(Human)