IDH3A Antibody - BSA Free

Western Blot: IDH3A Antibody [NBP2-16916] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.

Immunohistochemistry-Paraffin: IDH3A Antibody [NBP2-16916] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Western Blot: IDH3A Antibody [NBP2-16916] - 50 ug rat brain lysate/extract 10 % SDS-PAGE IDH3A antibody dilution: 1:10000.

Immunohistochemistry: IDH3A Antibody [NBP2-16916] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using IDH3A antibody (NBP2-16916) at 1:300 dilution.

Western Blot: IDH3A Antibody [NBP2-16916] - Sample (30 ug of whole cell lysate) A: whole zebrafish B: zebrafish heart 10% SDS PAGE NBP2-16916 diluted at 1:1000

Western Blot: IDH3A Antibody [NBP2-16916] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (NBP2-16916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IDH3A Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human IDH3A. The exact sequence is proprietary.
• Localization: Mitochondrion
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IDH3A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for IDH3A Antibody - BSA Free

• EC 1.1.1
• EC 1.1.1.41
• H-IDH alpha
• isocitrate dehydrogenase (NAD+) alpha chain
• isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
• isocitrate dehydrogenase 3 (NAD+) alpha
• Isocitric dehydrogenase subunit alpha
• NAD(+)-specific ICDH subunit alpha
• NAD(H)-specific isocitrate dehydrogenase alpha subunit
• NAD+-specific ICDH

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: IDH3A
• Uniprot:
  • P50213(Human)