IDH3A Antibody

Images

 
Western Blot: IDH3A Antibody [NBP2-16916] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: IDH3A Antibody [NBP2-16916] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
Western Blot: IDH3A Antibody [NBP2-16916] - 50 ug rat brain lysate/extract 10 % SDS-PAGE IDH3A antibody dilution: 1:10000.
Western Blot: IDH3A Antibody [NBP2-16916] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.63 mg/ml

Order Details

IDH3A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IDH3A. The exact sequence is proprietary.
Localization
Mitochondrion
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDH3A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.63 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IDH3A Antibody

  • EC 1.1.1
  • EC 1.1.1.41
  • H-IDH alpha
  • isocitrate dehydrogenase (NAD+) alpha chain
  • isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
  • isocitrate dehydrogenase 3 (NAD+) alpha
  • Isocitric dehydrogenase subunit alpha
  • NAD(+)-specific ICDH subunit alpha
  • NAD(H)-specific isocitrate dehydrogenase alpha subunit
  • NAD+-specific ICDH

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol IDH3A
Uniprot