IDH2 Antibody

Images

 
Orthogonal Strategies: Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody [NBP1-85784] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining in human heart muscle and testis tissues using anti-IDH2 antibody. Corresponding IDH2 RNA-seq data are presented for the ...read more
Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in human cell line MOLT-4.
Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human testis shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

IDH2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: REPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFD
Specificity
Specificity of human, mouse, rat IDH2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Liver Lysate (NB820-59232)
Control Peptide
IDH2 Protein (NBP1-85784PEP)
Publications
Read Publication using
NBP1-85784 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25643707).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IDH2 Antibody

  • D2HGA2
  • EC 1.1.1.42
  • ICD-M
  • IDH
  • IDHM
  • IDP
  • IDPM
  • isocitrate dehydrogenase [NADP], mitochondrial
  • isocitrate dehydrogenase 2 (NADP+), mitochondrial
  • mNADP-IDH
  • NADP(+)-specific ICDH
  • Oxalosuccinate decarboxylase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IDH2 Antibody (NBP1-85784)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Mouse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for IDH2 Antibody (NBP1-85784)

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Bioinformatics

Gene Symbol IDH2
Entrez
Uniprot