Orthogonal Strategies: Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody [NBP1-85784] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining in human heart muscle and testis tissues using anti-IDH2 antibody. Corresponding IDH2 RNA-seq data are presented for the ...read more
Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in human cell line MOLT-4.
Western Blot: IDH2 Antibody [NBP1-85784] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: IDH2 Antibody [NBP1-85784] - Staining of human testis shows low expression as expected.
This antibody was developed against Recombinant Protein corresponding to amino acids: REPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFD
Specificity of human, mouse, rat IDH2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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