ID1 Antibody (PCRP-ID1-2F11)

Immunocytochemistry/Immunofluorescence: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ID1 Antibody (PCRP-ID1-2F11). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940] - Flow cytometric analysis of PFA-fixed HeLa cells. ID1 antibody (PCRP-ID1-2F11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: ID1 Antibody (PCRP-ID1-2F11) [NBP3-13940] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ID1 Antibody (PCRP-ID1-2F11).

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IP, MA
• Clone: PCRP-ID1-2F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ID1 Antibody (PCRP-ID1-2F11) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14075)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant fragment (aa 5-150) of human ID1 (Uniprot: P41134)
• Localization: Nucleus, Cytoplasm
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ID1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 16.13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for ID1 Antibody (PCRP-ID1-2F11)

• BHLHB24
• bHLHb24DNA-binding protein inhibitor ID-1
• Class B basic helix-loop-helix protein 24
• dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein)
• dJ857M17.1.2
• ID
• ID1
• inhibitor of differentiation 1
• Inhibitor of DNA binding 1
• inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Background

Id1 is encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ID1
• Uniprot:
  • P41134(Human)