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ICOS Antibody (669222) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
669222
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6975U-100UG
Alexa Fluor 405
FAB6975V-100UG
Alexa Fluor 488
FAB6975G-100UG
Alexa Fluor 647
FAB6975R-100UG
Alexa Fluor 700
FAB6975N-100UG
Alexa Fluor 750
FAB6975S-100UG
Allophycocyanin
FAB6975A
Phycoerythrin
FAB6975P
Unconjugated
MAB6975
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6975
Carrier Free
MAB6975
Carrier Free
FAB6975P
Carrier Free
FAB6975A
Carrier Free
MAB6975-SP
Carrier Free
FAB6975V-100UG
Carrier Free
FAB6975U-100UG
Carrier Free
FAB6975S-100UG
Carrier Free
FAB6975R-100UG
Carrier Free
FAB6975N-100UG
Carrier Free
FAB6975G-100UG
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ICOS Antibody (669222) [Alexa Fluor® 594] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail
Glu21-Phe141
Accession # Q9Y6W8
Specificity
Detects human ICOS in direct ELISAs.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ICOS
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICOS Antibody (669222) [Alexa Fluor® 594]

  • Activation-inducible lymphocyte immunomediatory molecule
  • AILIM
  • AILIMCD278
  • CD278 antigen
  • CD278
  • CRP-1
  • CVID1
  • ICOS
  • inducible costimulator
  • inducible T-cell costimulator
  • inducible T-cell co-stimulator
  • MGC39850

Background

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB6975U
Alexa Fluor 405 FAB6975V
Alexa Fluor 488 FAB6975G
Alexa Fluor 594 FAB6975T
Alexa Fluor 647 FAB6975R
Alexa Fluor 700 FAB6975N
Alexa Fluor 750 FAB6975S
Allophycocyanin FAB6975A
Phycoerythrin FAB6975P
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ICOS
Uniprot