Recombinant Human ICEBERG Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human ICEBERG Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-90 of Human ICEBERG

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL VTGKGPKSCC KFIKHLCEED PQLASKMGLH

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CARD18
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
12.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol, 0.1M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human ICEBERG Protein

  • caspase recruitment domain family, member 18
  • Caspase-1 inhibitor Iceberg
  • ICEBERG caspase-1 inhibitor
  • ICEBERGcaspase recruitment domain-containing protein 18
  • pseudo-ICE
  • UNQ5804

Background

Caspase recruitment domain-containing protein 18, also known as CARD18, is a member of the death-domain-fold superfamily that is induced by proinflammatory stimuli. It consists essentially only of a CARD, inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. The association of CARD18 with caspase-1 is facilitated by the charge-charge interactions between the prodomain of caspase-1 and the surface charge of CARD18. Recombinant human CARD18 proetin, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CARD18