COP Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary ...read more
Flow Cytometry: COP Antibody [NB100-856] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

COP Antibody Summary

Immunogen
Peptide with sequence C-ETLGLSAGPIPGN corresponding to C-Terminus according to NP_443121.1.
Specificity
This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CARD16
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 28-30 kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.7 kDa band according to NP_443121.1. The 28-30 kDa band was successfully blocked by incubation with the immunizing peptide.
Control
COP Overexpression Lysate
Publications
Read Publication using NB100-856.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for COP Antibody

  • CARD only domain-containing protein 1
  • CARD only protein
  • caspase recruitment domain family, member 16
  • Caspase-1 inhibitor COP
  • COP1caspase-1 dominant-negative inhibitor pseudo-ICE
  • COPcaspase recruitment domain-containing protein 16
  • EC 3.4.22.36
  • Pseudo interleukin-1 beta converting enzyme
  • pseudo interleukin-1beta converting enzyme
  • PSEUDO-ICE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COP Antibody (NB100-856)(1)

Reviews for COP Antibody (NB100-856) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for COP Antibody (NB100-856) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional COP Products

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Blogs on COP

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Calculators

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Bioinformatics

Gene Symbol CARD16
Entrez
Uniprot