ICAP-1 Antibody [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 532

ICAP-1 Antibody [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human ICAP-1
  Met1-Tyr127
  Accession # O14713
• Specificity: Detects human ICAP-1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAP-1 Antibody [Alexa Fluor® 532]

• Bodenin
• DKFZp686K08158
• ICAP1
• ICAP-1
• ICAP-1alpha
• ICAP-1B
• ICAP1ICAP1AICAP1BICAP-1A
• integrin beta 1 binding protein 1
• integrin beta-1-binding protein 1
• Integrin cytoplasmic domain-associated protein 1
• integrin cytoplasmic domain-associated protein 1-alpha
• integrin cytoplasmic domain-associated protein 1-beta
• ITGB1BP1

Background

ICAP-1 (Integrin cytoplasmic domain-associated protein-1; also ITBP1) is a 22-27 kDa protein that specifically binds to the cytoplasmic tail of beta 1 integrin. It is widely expressed, and serves to negatively regulate cell migration/proliferation. In the cytosol, it has at least two functions. First, it associates with beta 1 integrin, promoting an inactive conformation. This has the effect of both slowing cell migration and allowing for the monitoring of ECM density. Second, it induces Delta-Notch signaling, an event that specifies the growth of only tip endothelium during angiogenesis. In the nucleus, ICAP-1 is proposed to participate in c-myc activation. Human ICAP-1 is 200 amino acids (aa) in length. It contains an NLS (aa 5-9) and one pleckstrin homology-like/PID domain (aa 58-200). Phosphorylation occurs at Thr38 and Ser41. There is one nonintegrin-binding alternative splice form that shows a deletion of aa 128-177. Over aa 1-127, human ICAP-1 shares 93% aa identity with mouse ICAP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.