ICAP-1 Antibody

Western blot shows lysates of human small intestine tissue, human testis tissue, human spleen tissue, and human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human ICAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ICAP-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human ICAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6805) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ICAP-1 Antibody Summary

• Immunogen: E. coli-derived recombinant human ICAP-1
  Met1-Tyr127
  Accession # O14713
• Specificity: Detects human ICAP-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ITGB1BP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAP-1 Antibody

• Bodenin
• DKFZp686K08158
• ICAP1
• ICAP-1
• ICAP-1alpha
• ICAP-1B
• ICAP1ICAP1AICAP1BICAP-1A
• integrin beta 1 binding protein 1
• integrin beta-1-binding protein 1
• Integrin cytoplasmic domain-associated protein 1
• integrin cytoplasmic domain-associated protein 1-alpha
• integrin cytoplasmic domain-associated protein 1-beta
• ITGB1BP1

Background

ICAP-1 (Integrin cytoplasmic domain-associated protein-1; also ITBP1) is a 22-27 kDa protein that specifically binds to the cytoplasmic tail of beta 1 integrin. It is widely expressed, and serves to negatively regulate cell migration/proliferation. In the cytosol, it has at least two functions. First, it associates with beta 1 integrin, promoting an inactive conformation. This has the effect of both slowing cell migration and allowing for the monitoring of ECM density. Second, it induces Delta-Notch signaling, an event that specifies the growth of only tip endothelium during angiogenesis. In the nucleus, ICAP-1 is proposed to participate in c-myc activation. Human ICAP-1 is 200 amino acids (aa) in length. It contains an NLS (aa 5-9) and one pleckstrin homology-like/PID domain (aa 58-200). Phosphorylation occurs at Thr38 and Ser41. There is one nonintegrin-binding alternative splice form that shows a deletion of aa 128-177. Over aa 1-127, human ICAP-1 shares 93% aa identity with mouse ICAP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAP-1 Antibody (AF6805)(2)

Publications using AF6805

• VL Su, B Simon, KM Draheim, DA Calderwood Serine phosphorylation of the small phosphoprotein ICAP1 inhibits its nuclear accumulation J. Biol. Chem., 2020-01-31;0(0):. 2020-01-31 [PMID: 32005669] (Western Blot, Human)
• Kyle M Draheim Nuclear Localization of Integrin Cytoplasmic Associated Protein-1 Influences ?1 Integrin Activation and Recruits Krev/ Interaction Trapped-1 to the Nucleus J. Biol. Chem, 2016-12-21;0(0):. 2016-12-21 [PMID: 28003363] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ITGB1BP1
• Uniprot:
  • O14713(Human)