Human ICAM-3/CD50 ELISA Kit (Colorimetric)

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Human ICAM-3/CD50 ELISA Kit (Colorimetric) [NBP1-89872] - Standard curve using Human ICAM-3/CD50 ELISA Kit (Colorimetric).

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-89872
    • Availability
      Product Discontinued

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Human ICAM-3/CD50 ELISA Kit (Colorimetric) Summary

Description
The human sICAM-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sICAM-3
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human ICAM-3 positive serum. There was no crossreactivity detected, notably not with human sICAM-1.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 2.0%
Intra-Assay
CV% < 2.5%
Spike Recovery
The recovery ranged from 98% to 112% with an overall mean recovery of 106%. Please see protocol for more specific Spike Recovery details.
Sample Volume
20 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ICAM3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-3
  3. HRP-Conjugate anti-human sICAM-3 monoclonal antibody
  4. Human sICAM-3 Standard, 50 ng/ml
  5. Sample Diluent
  6. Stop Solution (1M Phosphoric acid)
  7. Substrate Solution (tetramethyl-benzidine)
  8. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human ICAM-3/CD50 ELISA Kit (Colorimetric)

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Background

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for ICAM-3/CD50 ELISA Kit (NBP1-89872). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol ICAM3