Human ICAM-3/CD50 ELISA Kit (Colorimetric) Summary
Description |
The human sICAM-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sICAM-3 |
Specificity |
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human ICAM-3 positive serum. There was no crossreactivity detected, notably not with human sICAM-1. |
Standard Curve Range |
0.78 - 50 ng/mL |
Sensitivity |
0.38 ng/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 2.0% |
Intra-Assay |
CV% < 2.5% |
Spike Recovery |
The recovery ranged from 98% to 112% with an overall mean recovery of 106%. Please see protocol for more specific Spike Recovery details. |
Sample Volume |
20 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ICAM3 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-3
- HRP-Conjugate anti-human sICAM-3 monoclonal antibody
- Human sICAM-3 Standard, 50 ng/ml
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
|
Alternate Names for Human ICAM-3/CD50 ELISA Kit (Colorimetric)
Background
All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for ICAM-3/CD50 ELISA Kit (NBP1-89872). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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