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Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid, urine

Order Details

ICAM-3/CD50 ELISA Kit Summary

Standard Curve Range
0.78 - 50 ng/mL
0.38 ng/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.


Application Notes
The human sICAM-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sICAM-3

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-3
  2. HRP-Conjugate anti-human sICAM-3 monoclonal antibody
  3. Human sICAM-3 Standard, 50 ng/ml
  4. Sample Diluent
  5. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  6. Substrate Solution (tetramethyl-benzidine)
  7. Stop Solution (1M Phosphoric acid)
  8. Adhesive Films

Reactivity Notes



Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for ICAM-3/CD50 ELISA Kit

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3


ICAM-3 is a heavily glycosylated, integral membrane protein with five immunoglobulin-like domains. It shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses. The initial interaction of ICAM-3 with LFA-1 might increase LFA-1-mediated cell binding to ICAM-1. Furthermore, ICAM-3 expression has been shown for dendritic epidermal Langerhans cells, whereas it is absent on other dendritic cells from different lymphoid organs. Thus potential function of ICAM-3 at the initiation phase of LC-leukocyte interactions taking place during skin localized immune reactions can be postulated.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Contact Information

Product PDFs


Gene Symbol ICAM3

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