Human ICAM-3/CD50 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid, urine
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.38 ng/mL

Order Details

Human ICAM-3/CD50 ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human ICAM-3 positive serum. There was no crossreactivity detected, notably not with human sICAM-1.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
2.0%
Intra-Assay
2.5%
Spike Recovery
The recovery ranged from 98% to 112% with an overall mean recovery of 106%. Please see protocol for more specific Spike Recovery details.
Sample Volume
20 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ICAM3

Applications/Dilutions

Application Notes
The human sICAM-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sICAM-3

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Sample Diluent
  2. Stop Solution (1M Phosphoric acid)
  3. Substrate Solution (tetramethyl-benzidine)
  4. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  5. Human sICAM-3 Standard, 50 ng/ml
  6. HRP-Conjugate anti-human sICAM-3 monoclonal antibody
  7. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-3
  8. Adhesive Films

Alternate Names for Human ICAM-3/CD50 ELISA Kit (Colorimetric)

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ICAM3
Entrez