Human ICAM-3/CD50 ELISA Kit (Colorimetric)

Human ICAM-3/CD50 ELISA Kit (Colorimetric) [NBP1-89872] - Standard curve using Human ICAM-3/CD50 ELISA Kit (Colorimetric).

Product Discontinued

Human ICAM-3/CD50 ELISA Kit (Colorimetric) Summary

• Description: The human sICAM-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sICAM-3
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human ICAM-3 positive serum. There was no crossreactivity detected, notably not with human sICAM-1.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.38 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 2.0%
• Intra-Assay: CV% < 2.5%
• Spike Recovery: The recovery ranged from 98% to 112% with an overall mean recovery of 106%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 20 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ICAM3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ICAM-3/CD50 ELISA Kit (Colorimetric)

• CD50 antigen
• CD50
• CDW50CDw50
• ICAM3
• ICAM-3
• ICAM-Rintercellular adhesion molecule-3
• intercellular adhesion molecule 3

Background

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for ICAM-3/CD50 ELISA Kit (NBP1-89872). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ICAM3