ICAM-3/CD50 Antibody (Cal 3.34) [HRP]

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: Cal 3.34
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

ICAM-3/CD50 Antibody (Cal 3.34) [HRP] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Recombinant human ICAM‑3/CD50 Fc Chimera
• Specificity: Detects human ICAM‑3/CD50 in Western blots. ICAM‑3/CD50 was found not to recognize CHO cells expressing the human ICAM-1 protein. ICAM‑3/CD50 was found to recognize CHO cells expressing the human ICAM-3 protein. The epitope for ICAM‑3/CD50 is located on domain 4 of the human ICAM-3 molecule.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM3
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product BBA28. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for ICAM-3/CD50 Antibody (Cal 3.34) [HRP]

• CD50 antigen
• CD50
• CDW50CDw50
• ICAM3
• ICAM-3
• ICAM-Rintercellular adhesion molecule-3
• intercellular adhesion molecule 3

Background

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ICAM3