ICAM-3/CD50 Antibody (Cal 3.10) [mFluor Violet 450 SE]

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clone: Cal 3.10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 450 SE

ICAM-3/CD50 Antibody (Cal 3.10) [mFluor Violet 450 SE] Summary

• Immunogen: Recombinant human ICAM-3/CD50 Fc Chimera
• Specificity: Detects human ICAM-3/CD50 in Western blots. Does not cross-react with recombinant human (rh) ICAM-1 or rhVCAM-1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM3
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ICAM-3/CD50 Antibody (Cal 3.10) [mFluor Violet 450 SE]

• CD50 antigen
• CD50
• CDW50CDw50
• ICAM3
• ICAM-3
• ICAM-Rintercellular adhesion molecule-3
• intercellular adhesion molecule 3

Background

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ICAM3