ICAM-3/CD50 Antibody (186-2G9)

Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (186-2G9) [NBP2-32941] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (186-2G9).

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: 186-2G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ICAM-3/CD50 Antibody (186-2G9) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34521)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Clone 186-2G9 was used by HLDA to establish CD designation.
• Immunogen: Stimulated human leukocytes
• Localization: Cell surface
• Specificity: Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3). Order Ab without Azide.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for ICAM-3/CD50 Antibody (186-2G9)

• CD50 antigen
• CD50
• CDW50CDw50
• ICAM3
• ICAM-3
• ICAM-Rintercellular adhesion molecule-3
• intercellular adhesion molecule 3

Background

All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM 3 is the major ligand for LFA-1 in the resting state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ICAM3
• Uniprot:
  • P32942(Human)