IBSP/Sialoprotein II Antibody

Western Blot: Sialoprotein II Antibody [NBP1-70422] - Titration: 1.25ug/ml, Positive Control: HepG2 cell lysate.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

IBSP/Sialoprotein II Antibody Summary

• Immunogen: Synthetic peptides corresponding to IBSP (integrin-binding sialoprotein (bone sialoprotein, bone sialoprotein II)) The peptide sequence was selected from the N terminal of IBSP. Peptide sequence SATTLGYGEDATPGTGYTGLAAIQLPKKAGDITNKATKEKESDEEEEEEE The peptide sequence for this immunogen was taken from within the described region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IBSP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 ug/ml
• Application Notes: This is a rabbit polyclonal antibody against IBSP and was validated on Western blot.
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for IBSP/Sialoprotein II Antibody

• BNSP
• Bone sialoprotein 2
• Bone sialoprotein
• BSP 2
• BSP II
• BSP
• BSP2
• BSPII
• BSP-II
• Cell binding sialoprotein
• IBSP
• Integrin binding sialoprotein
• SP II
• SPII
• SP-II

Background

The protein encoded by the IBSP gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IBSP/Sialoprotein II Antibody (NBP1-70422) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Catrina Alarcon IHC-Fr Mouse 02/03/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse long bone and knee joint,Mouse proximal tibia
• Species: Mouse
• Lot: QC5709-41929

Comments

• Comments: Our previous studies have shown that BSPII has high expression in the epiphysis of the developing mouse proximal tibia and distal femur. To visualize this, we stained the tissue with BSPII antibody (NBP1-70422). As it was tested to work in human tissue and with western blots and the fact that there were not many other antibody options with Novus, we attempted the same with mouse tissue. We tested both 1:50 and 1:100 concentrations due to the higher concentrations typically needed when performing IHC vs Western blot. Unfortunately our stains came up negative/unspecific. This data was controlled for by another BSPII antibody we obtained from another vendor years ago.

Bioinformatics

• Gene Symbol: IBSP