IBSP/Sialoprotein II Antibody

Images

 
Western Blot: Sialoprotein II Antibody [NBP1-70422] - Titration: 1.25ug/ml, Positive Control: HepG2 cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

IBSP/Sialoprotein II Antibody Summary

Immunogen
Synthetic peptides corresponding to IBSP (integrin-binding sialoprotein (bone sialoprotein, bone sialoprotein II)) The peptide sequence was selected from the N terminal of IBSP. Peptide sequence SATTLGYGEDATPGTGYTGLAAIQLPKKAGDITNKATKEKESD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IBSP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against IBSP and was validated on Western blot.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 1
using
NBP1-70422 in the following application:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for IBSP/Sialoprotein II Antibody

  • BNSP
  • Bone sialoprotein 2
  • Bone sialoprotein
  • BSP 2
  • BSP II
  • BSP
  • BSP2
  • BSPII
  • BSP-II
  • Cell binding sialoprotein
  • IBSP
  • Integrin binding sialoprotein
  • SP II
  • SPII
  • SP-II

Background

The protein encoded by the IBSP gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
MAB1419
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
NB100-56630
Species: Hu
Applications: WB
NB110-3638
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NBP1-89131
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-42378
Species: Hu
Applications: WB, IHC
NBP2-32263
Species: Hu
Applications: WB, IHC, IP
NBP1-89134
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-91612
Species: Mu
Applications: WB
AF942
Species: Mu
Applications: WB, IHC, CyTOF-ready, ICFlow
NBP1-45525
Species: Hu, Mu, Rt
Applications: WB
355-BM/CF
Species: Hu, Mu, Rt
NBP2-38019
Species: Hu
Applications: IHC, IHC-P
H00009988-M03
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-91258
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-70422
Species: Hu
Applications: WB

Publications for IBSP/Sialoprotein II Antibody (NBP1-70422) (0)

There are no publications for IBSP/Sialoprotein II Antibody (NBP1-70422).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for IBSP/Sialoprotein II Antibody (NBP1-70422) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NBP1-70422:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen IBSP/Sialoprotein II NBP1-70422
Enlarge
reviewed by:
Catrina Alarcon
IHC-Fr Mouse 02/03/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse long bone and knee joint,Mouse proximal tibia
SpeciesMouse
LotQC5709-41929

Comments

CommentsOur previous studies have shown that BSPII has high expression in the epiphysis of the developing mouse proximal tibia and distal femur. To visualize this, we stained the tissue with BSPII antibody (NBP1-70422). As it was tested to work in human tissue and with western blots and the fact that there were not many other antibody options with Novus, we attempted the same with mouse tissue. We tested both 1:50 and 1:100 concentrations due to the higher concentrations typically needed when performing IHC vs Western blot. Unfortunately our stains came up negative/unspecific. This data was controlled for by another BSPII antibody we obtained from another vendor years ago.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IBSP/Sialoprotein II Antibody (NBP1-70422) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IBSP/Sialoprotein II Products

Bioinformatics Tool for IBSP/Sialoprotein II Antibody (NBP1-70422)

Discover related pathways, diseases and genes to IBSP/Sialoprotein II Antibody (NBP1-70422). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IBSP/Sialoprotein II Antibody (NBP1-70422)

Discover more about diseases related to IBSP/Sialoprotein II Antibody (NBP1-70422).
 

Pathways for IBSP/Sialoprotein II Antibody (NBP1-70422)

View related products by pathway.

PTMs for IBSP/Sialoprotein II Antibody (NBP1-70422)

Learn more about PTMs related to IBSP/Sialoprotein II Antibody (NBP1-70422).

Blogs on IBSP/Sialoprotein II

There are no specific blogs for IBSP/Sialoprotein II, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

1
5
0
4
0
3
0
2
0
1
1

Catrina Alarcon
02/03/2017
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol IBSP
Entrez