Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3)

Western Blot: HAO1 Antibody (5C3) [NBP2-03361] Analysis of extracts (35ug) from 9 different cell lines by using anti-HAO1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow Cytometry: HAO1 Antibody (5C3) [NBP2-03361] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HAO1 antibody, and then analyzed by flow cytometry.

Western Blot: HAO1 Antibody (5C3) [NBP2-03361] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAO1.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow
• Clone: OTI5C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.75 mg/ml

Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3) Summary

• Immunogen: Full length human recombinant protein of human HAO1 (NP_060015) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Western Blot 1:1000-2000
• Theoretical MW: 40.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.75 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Hydroxyacid Oxidase-1/HAO-1 Antibody (OTI5C3)

• (S)-2-hydroxy-acid oxidase
• EC 1.1.3.15
• Glycolate oxidase
• GOX
• GOX1MGC142227
• GOXMGC142225
• HAO1
• HAO-1
• HAOX1
• hydroxyacid oxidase (glycolate oxidase) 1
• hydroxyacid oxidase 1
• Hydroxyacid Oxidase1
• Hydroxyacid Oxidase-1

Background

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq]. COMPLETENESS: full length.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAO1
• Entrez:
  • 54363(Human)