Hyaluronan synthase 1 Antibody (3E10)

Western Blot: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis using HAS1 mAb against human HAS1 (AA: 74-243) recombinant protein. (Expected MW is 44.4 kDa)

Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (3E10) [NBP1-51635] - Analysis of U251 cells using HAS1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Hyaluronan synthase 1 Antibody (3E10) Summary

• Description: Novus Biologicals Mouse Hyaluronan synthase 1 Antibody (3E10) (NBP1-51635) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Hyaluronan synthase 1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Hyaluronan synthase 1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAS1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Hyaluronan synthase 1 Antibody (3E10)

• HA synthase 1
• HAS
• huHAS1
• hyaluronan synthase 1
• hyaluronate synthase 1
• hyaluronic acid synthase 1

Background

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. HA is synthesized by membrane-bound HA synthases (HAS1, HAS2 and HAS3) at the inner surface of the plasma membrane. The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix. The production of HA by HASs has been implicated in heart development, angiogenesis, and stem cell biology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hyaluronan synthase 1 Antibody (NBP1-51635)(3)

Publications using NBP1-51635

• Solier S, Müller S, Cañeque T et al. A druggable copper-signalling pathway that drives inflammation Nature 2023-04-26 [PMID: 37100912] (IHC, Human)
• Cheng AM, Yin HY, Chen A et al. Celecoxib and Pioglitazone as Potential Therapeutics for Regulating TGF-beta-Induced Hyaluronan in Dysthyroid Myopathy. Invest. Ophthalmol. Vis. Sci. 2016-04-01 [PMID: 27092721] (WB, Human)
• Saito S, Takayama Y, Mizumachi K, Suzuki C. Lactoferrin promotes hyaluronan synthesis in human dermal fibroblasts Biotechnol Lett 2011-01-01 [PMID: 20820878] (WB, Human)

Bioinformatics

• Gene Symbol: HAS1
• Entrez:
  • 3036(Human)
• Uniprot:
  • Q92839(Human)